Schejter E D, Segal D, Glazer L, Shilo B Z
Cell. 1986 Sep 26;46(7):1091-101. doi: 10.1016/0092-8674(86)90709-9.
cDNA clones of the Drosophila epidermal growth factor receptor homolog (DER) gene were isolated and sequenced. The deduced amino acid sequence shows a similar degree of homology to the human epidermal growth factor receptor and to the rat and human neu proteins; the most striking difference is the addition of a third cysteine-rich extracellular domain in DER. The structure of the cDNA indicates the use of alternative 5' exons. Thus, the gene encodes three putative proteins differing at their N termini. The distribution of DER transcripts was analyzed by in situ hybridization. Transcripts are uniformly distributed in embryos, larval transcripts are primarily localized to proliferating tissues of the imaginal discs and brain cortex, and adult transcripts are detected mainly in the brain and ganglia. All three splicing alternatives show similar tissue distribution during development.
分离并测序了果蝇表皮生长因子受体同源物（DER）基因的cDNA克隆。推导的氨基酸序列显示出与人类表皮生长因子受体以及大鼠和人类neu蛋白相似程度的同源性；最显著的差异是DER中添加了第三个富含半胱氨酸的细胞外结构域。cDNA的结构表明使用了可变的5'外显子。因此，该基因编码三种推定的蛋白质，它们的N末端不同。通过原位杂交分析了DER转录本的分布。转录本在胚胎中均匀分布，幼虫转录本主要定位于成虫盘和大脑皮层的增殖组织，而成虫转录本主要在大脑和神经节中检测到。在发育过程中，所有三种剪接变体都显示出相似的组织分布。
