Lusson J, Vieau D, Hamelin J, Day R, Chrétien M, Seidah N G
J. A. DeSève Laboratorie of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, PQ, Canada.
Proc Natl Acad Sci U S A. 1993 Jul 15;90(14):6691-5. doi: 10.1073/pnas.90.14.6691.
By using reverse transcriptase/PCR and oligonucleotide sequences derived from conserved segments (including the conserved RRGDL sequence) of the known proprotein convertases (PCs) PC1, PC2, furin, and PC4, we identified a subtilisin/kexin-like PC called PC5 in both mouse and rat tissues. The composite structure (2.85 kb) was deduced from the analysis of the reverse transcription/PCR products combined with the sequence from a clone isolated from a cDNA library made from corticotropin-activated mouse adrenocortical Y1 cells. The deduced cDNA structures of mouse PC5 and rat PC5 showed that the closest homologue is PACE4. Furthermore, like furin, Drosophila melanogaster (d) dfurin2, and PACE4, PC5 shows the presence of a C-terminal Cys-rich domain containing either 5 (PC5 and PACE4) or 10 (dfurin2) repeats of the consensus motif Cys-Xaa2-Cys-Xaa3-Cys-Xaa(5-7)-Cys-Xaa2-Cys-Xaa (8-15)-Cys-Xaa3-Cys-Xaa(9-16). The richest sources of rat PC5 mRNA (3.8 kb) are the adrenal and gut, but it can also be detected in many endocrine and nonendocrine tissues. Corticotropin-stimulated adrenocortical Y1 cells showed an increased expression of PC5 mRNA, suggesting an upregulation by cAMP. In situ hybridization of rat brain sections demonstrated a unique distribution of PC5 compared to PC1, PC2, and furin.
通过使用逆转录酶/聚合酶链反应（RT/PCR）以及从已知前蛋白转化酶（PCs）PC1、PC2、弗林蛋白酶和PC4的保守区段（包括保守的RRGDL序列）衍生而来的寡核苷酸序列，我们在小鼠和大鼠组织中鉴定出一种枯草杆菌蛋白酶/克新样PC，称为PC5。通过对逆转录/PCR产物的分析，并结合从促肾上腺皮质激素激活的小鼠肾上腺皮质Y1细胞构建的cDNA文库中分离出的一个克隆的序列，推导出了该复合结构（2.85 kb）。小鼠PC5和大鼠PC5推导的cDNA结构表明，其最接近的同源物是PACE4。此外，与弗林蛋白酶、果蝇（d）dfurin2和PACE4一样，PC5显示在其C末端存在一个富含半胱氨酸的结构域，该结构域含有5个（PC5和PACE4）或10个（dfurin2）共有基序Cys-Xaa2-Cys-Xaa3-Cys-Xaa(5-7)-Cys-Xaa2-Cys-Xaa(8-15)-Cys-Xaa3-Cys-Xaa(9-16)的重复序列。大鼠PC5 mRNA（3.8 kb）最丰富的来源是肾上腺和肠道，但在许多内分泌和非内分泌组织中也能检测到。促肾上腺皮质激素刺激的肾上腺皮质Y1细胞显示PC5 mRNA表达增加，提示其受环磷酸腺苷（cAMP）上调。大鼠脑切片的原位杂交显示，与PC1、PC2和弗林蛋白酶相比，PC5具有独特的分布。
