Department of Life Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel.
The National Institute for Biotechnology in the Negev, Ben-Gurion University of the Negev, Beer-Sheva, Israel.
Front Endocrinol (Lausanne). 2022 Dec 7;13:1059936. doi: 10.3389/fendo.2022.1059936. eCollection 2022.
The giant freshwater prawn pj is one of the best studied species in aquaculture. However, the transcriptional changes associated with embryonic development and the sexual differentiation mechanism of remain to be elucidated. To characterize the embryonic development of this prawn and to determine whether differential expression and differential splicing play roles in the early sexual differentiation of , we profiled five developmental days of male and female embryos by RNA sequencing. We identified modules of co-expressed genes representing waves of transcription that correspond to physiological processes in early embryonic development (such as the maternal-to-zygotic transition) up to preparation for life outside the egg (development of muscles, cuticle etc.). Additionally, we found that hundreds of genes are differentially expressed between sexes, most of them uncharacterized, suggesting that the sex differentiation mechanism of might contain clade-specific elements. The resulting first-of-a-kind transcriptional map of embryonic development of male and female will guide future studies to reveal the roles of specific genes and splicing isoforms in the embryonic development and sexual differentiation process of .
巨型淡水虾 pj 是水产养殖中研究最深入的物种之一。然而，与胚胎发育相关的转录变化和性分化机制仍有待阐明。为了描述这种虾的胚胎发育，并确定差异表达和差异剪接是否在早期性分化中起作用，我们通过 RNA 测序对雄性和雌性胚胎的五个发育日进行了分析。我们确定了代表转录波的共表达基因模块，这些转录波对应于早期胚胎发育（如母体到合子的过渡）的生理过程，直到为离开卵外的生活做准备（肌肉、外骨骼等的发育）。此外，我们发现数百个基因在性别之间存在差异表达，其中大多数尚未被描述，这表明 的性分化机制可能包含特定进化枝的元素。这是首次对雄性和雌性 胚胎发育的转录图谱进行描述，将指导未来的研究揭示特定基因和剪接异构体在胚胎发育和性分化过程中的作用。
