Béganton Benoît, Coyaud Etienne, Mangé Alain, Solassol Jérôme
Département de pathologie et oncobiologie, Laboratoire de biologie des tumeurs solides, CHU de Montpellier, Univ. Montpellier, CHU Arnaud de Villeneuve, avenue du Doyen Giraud, 34295 Montpellier, France Institut de Recherche en Cancérologie de Montpellier (IRCM), Inserm U1194, Univ. Montpellier, 34070 Montpellier, France.
Princess Margaret Cancer Centre, University Health Network, Toronto, Canada.
Med Sci (Paris). 2019 Mar;35(3):223-231. doi: 10.1051/medsci/2019035. Epub 2019 Apr 1.
The proteome is a dynamic system in which protein-protein interactions play a crucial role to model together the cellular phenotype. However, given the inherent limitation of the available technologies to depict the dynamic nature of these interactions, identify protein-protein interaction has for a long time represented an important challenge in proteomic. The recent development of BioID and APEX, two proximity-dependent labeling technologies, opens today new perspectives and yet start changing our vision of protein-protein interaction, and more globally our vision of the proteome. In this review, we describe the recent and conventional tools available to study protein-protein interactions, compare the advantages and limitations of these technics, and discuss the recent progress brought by the proximity-dependent labelling to complete our vision of the proteome, and thus better understand cellular mechanisms.
蛋白质组是一个动态系统，其中蛋白质-蛋白质相互作用对于共同塑造细胞表型起着至关重要的作用。然而，鉴于现有技术在描述这些相互作用的动态性质方面存在固有限制，识别蛋白质-蛋白质相互作用长期以来一直是蛋白质组学中的一项重大挑战。BioID和APEX这两种邻近依赖性标记技术的最新发展，为我们开启了新的视角，也开始改变我们对蛋白质-蛋白质相互作用的看法，更广泛地说，改变了我们对蛋白质组的看法。在这篇综述中，我们描述了用于研究蛋白质-蛋白质相互作用的最新和传统工具，比较了这些技术的优缺点，并讨论了邻近依赖性标记在完善我们对蛋白质组的认识从而更好地理解细胞机制方面所取得的最新进展。
