Sugihara S, Kohno Y, Miyamoto S, Sasaki N, Niimi H, Nakajima H
Endocrinol Jpn. 1986 Jun;33(3):385-93. doi: 10.1507/endocrj1954.33.385.
The expression of activation antigens (transferrin receptor, IL-2 receptor and Ia antigen) on circulating T lymphocytes from Japanese children with Type 1 diabetes was studied using five monoclonal antibodies (Ab), OKT9, anti-Tac Ab, OKIa1, anti-human HLA-DR Ab and OKT3. For detecting Ia positive T cells, the dual staining technique using OKT3 and anti-Ia antibody was employed. Four out of six patients (67%) with newly diagnosed Type 1 diabetes showed a raised level of either OKT9 or Tac positive cells when examined at diagnosis. These patients, however, rapidly lost these activation antigens after the insulin therapy was started. In contrast, in 32 long-standing patients, only 2 (6%) had a high percentage of OKT9 positive cells and none of them demonstrated Tac positive cells. One out of six newly diagnosed patients or three out of 21 long-standing patients had a significantly high percentage of Ia-positive T cells compared with normal subjects. In poorly controlled long-standing patients whose HbA1 value was higher than 14%, none of them had an increased number of activated lymphocytes. Therefore, it is unlikely that insulin deficiency and hyperglycemia were responsible for the changes observed in these studies. Activated lymphocytes might be related to activation of the immune system involved in pathogenesis of Type 1 diabetes.
使用五种单克隆抗体（Ab），即OKT9、抗Tac Ab、OKIa1、抗人HLA - DR Ab和OKT3，研究了日本1型糖尿病儿童循环T淋巴细胞上活化抗原（转铁蛋白受体、IL - 2受体和Ia抗原）的表达。为检测Ia阳性T细胞，采用了使用OKT3和抗Ia抗体的双重染色技术。在六名新诊断的1型糖尿病患者中，有四名（67%）在诊断时检查显示OKT9或Tac阳性细胞水平升高。然而，这些患者在开始胰岛素治疗后迅速失去了这些活化抗原。相比之下，在32名病程较长的患者中，只有2名（6%）OKT9阳性细胞百分比高，且他们均未显示Tac阳性细胞。与正常受试者相比，六名新诊断患者中有一名，或21名病程较长患者中有三名Ia阳性T细胞百分比显著高。在HbA1值高于14%的控制不佳的病程较长患者中，他们均未出现活化淋巴细胞数量增加。因此，胰岛素缺乏和高血糖不太可能是这些研究中观察到的变化的原因。活化淋巴细胞可能与参与1型糖尿病发病机制的免疫系统激活有关。
