Department of Ophthalmology, Medical University of Innsbruck, Innsbruck, Austria.
Division of Hygiene and Medical Microbiology, Medical University of Innsbruck, Innsbruck, Austria.
Cornea. 2019 Aug;38(8):1011-1016. doi: 10.1097/ICO.0000000000001927.
N-chlorotaurine (NCT) is an anti-infective belonging to the class of chloramines and an investigative drug for the topical treatment of keratoconjunctivitis. The aim of the present study was to demonstrate its efficacy against Acanthamoeba and Candida in corneas infected ex vivo.
Corneal buttons from porcine eyes were contaminated with Acanthamoeba castellanii trophozoites or Candida albicans Centraalbureau voor Schimmelcultures 5982 and incubated for 7 and 3 days, respectively. Subsequently, they were treated with 1% NCT for 5 to 120 minutes. After further incubation for 2 days in the absence of NCT in tests with A. castellanii, the buttons were homogenized, and the amoebae grown for a further 5 days before they were counted in a light microscope. For C. albicans, quantitative cultures were performed from corneal homogenates.
Incubation of 120 minutes in NCT completely inhibited the regrowth of A. castellanii and reduced the number of C. albicans colony-forming unit counts by 4 log10. In addition, at 60 minutes, significant reductions of both pathogens could be observed. Histology showed penetration of pathogens into the stroma of the corneal buttons.
NCT inactivates A. castellanii and C. albicans in corneal tissue.
N-氯代牛磺酸（NCT）是一种属于氯胺类的抗感染药物，也是治疗角结膜炎的研究性药物。本研究旨在证明其对体外感染角膜的棘阿米巴原虫和白色念珠菌的疗效。
从猪眼采集角膜纽扣，并用棘阿米巴原虫滋养体或白色念珠菌 CBS 5982 污染，并分别孵育 7 天和 3 天。然后，用 1% NCT 处理 5 至 120 分钟。在用 A. castellanii 进行的试验中，在没有 NCT 的情况下进一步孵育 2 天后，将纽扣匀浆，并在光学显微镜下计数进一步生长 5 天的阿米巴虫。对于白色念珠菌，从角膜匀浆中进行定量培养。
NCT 孵育 120 分钟可完全抑制棘阿米巴原虫的再生，并将白色念珠菌的菌落形成单位计数减少 4 个对数。此外，在 60 分钟时，两种病原体的数量都明显减少。组织学显示病原体穿透了角膜纽扣的基质。
NCT 可使角膜组织中的棘阿米巴原虫和白色念珠菌失活。
