Gélinas P, Goulet J, Tastayre G M, Picard G A
Département de sciences et technologie des aliments and Centre de recherche en nutrition, Université Laval, Québec City, Quebec, Canada G1K 7P4 and Ecochimie Ltée, Longueuil, Québec, Canada J4G 1S5.
J Food Prot. 1984 Nov;47(11):841-847. doi: 10.4315/0362-028X-47.11.841.
The combined influence of temperature (4, 20, 37 and 50°C) and contact time (10, 20 and 30 min) on the efficacy of eight commercial disinfectants was evaluated by the Association of Official Analytical Chemists use-dilution method. An increase of temperature greatly enhanced the activity of all tested solutions, particularly glutaraldehyde, chlorhexidine acetate and the amphoteric surfactant, whereas contact time mainly enhanced the efficacy of sodium hypochlorite, the quaternary ammonium compound and the amphoteric surfactant. Temperature and contact time influenced the activity profile of the disinfectants tested, with a maximum efficacy near the optimum growth temperature (37°C) of the test organism ( Pseudomonas aeruginosa ATCC 15442). This organism was highly resistant to the amphoteric surfactant as well as to the two quaternary ammonium compounds. Classification of disinfectants is proposed on the basis of their mode of action, temperature dependence and activation energies, heat and light stability, and tolerance to organic matter.
采用美国官方分析化学师协会使用稀释法，评估了温度（4、20、37和50°C）和接触时间（10、20和30分钟）对八种商用消毒剂效力的综合影响。温度升高极大地增强了所有测试溶液的活性，尤其是戊二醛、醋酸氯己定和两性表面活性剂，而接触时间主要增强了次氯酸钠、季铵化合物和两性表面活性剂的效力。温度和接触时间影响了所测试消毒剂的活性特征，在测试微生物（铜绿假单胞菌ATCC 15442）的最佳生长温度（37°C）附近效力最高。该微生物对两性表面活性剂以及两种季铵化合物具有高度抗性。根据消毒剂的作用方式、温度依赖性和活化能、热稳定性和光稳定性以及对有机物的耐受性，对消毒剂进行了分类。
