Laboratory of Marine Chemical Resource Development, Faculty of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido 041-8611, Japan.
Chair of Marine Chemical Resource Development, Graduate School of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido 041-8611, Japan.
Mar Drugs. 2019 Mar 25;17(3):190. doi: 10.3390/md17030190.
Plastid proteins are one of the main components in red algae. In order to clarify the angiotensin I converting enzyme (ACE) inhibitory peptides from red alga sp. (Japan), we determined the plastid genome sequence. The genome possesses 205 protein coding genes, which were classified as genetic systems, ribosomal proteins, photosystems, adenosine triphosphate (ATP) synthesis, metabolism, transport, or unknown. After comparing ACE inhibitory peptides between protein sequences and a database, photosystems (177 ACE inhibitory peptides) were found to be the major source of ACE inhibitory peptides (total of 751). Photosystems consist of phycobilisomes, photosystem I, photosystem II, cytochrome complex, and a redox system. Among them, photosystem I (53) and II (51) were the major source of ACE inhibitory peptides. We found that the amino acid sequence of apcE (14) in phycobilisomes, psaA (18) and psaB (13) in photosystem I, and psbB (11) and psbC (10) in photosystem II covered a majority of bioactive peptide sequences. These results are useful for evaluating the bioactive peptides from red algae.
质体蛋白是红藻的主要成分之一。为了从红藻 sp.（日本）中阐明血管紧张素转化酶（ACE）抑制肽，我们测定了质体基因组序列。该基因组拥有 205 个蛋白质编码基因，这些基因被分类为遗传系统、核糖体蛋白、光合系统、三磷酸腺苷（ATP）合成、代谢、运输或未知。在比较蛋白质序列和数据库之间的 ACE 抑制肽后，发现光合系统（177 个 ACE 抑制肽）是 ACE 抑制肽的主要来源（共 751 个）。光合系统由藻胆体、光系统 I、光系统 II、细胞色素复合物和氧化还原系统组成。其中，光系统 I（53 个）和 II（51 个）是 ACE 抑制肽的主要来源。我们发现藻胆体中的 apcE（14）、光系统 I 中的 psaA（18）和 psaB（13）以及光系统 II 中的 psbB（11）和 psbC（10）的氨基酸序列涵盖了大多数生物活性肽序列。这些结果有助于评估红藻的生物活性肽。
