Department of Biotechnology, Quaid-i-Azam University, Islamabad-45320, Pakistan.
Laboratoire de Biologie des Ligneux et des Grandes Cultures (LBLGC), INRA USC1328, Université d'Orléans, 45067 Orléans CEDEX 2, France.
Molecules. 2019 Mar 27;24(7):1207. doi: 10.3390/molecules24071207.
(L.) Gaertn. is a well-known medicinal herb, primarily used in liver protection. Light strongly affects several physiological processes along with secondary metabolites biosynthesis in plants. Herein, was exploited for in vitro potential under different light regimes in the presence of melatonin. The optimal callogenic response occurred in the combination of 1.0 mg/L α-naphthalene acetic acid and 0.5 mg/L 6-benzylaminopurine under photoperiod. Continuous light associated with melatonin treatment increased total flavonoid content (TFC), total phenolic content (TPC) and antioxidant potential, followed by photoperiod and dark treatments. The increased level of melatonin has a synergistic effect on biomass accumulation under continuous light and photoperiod, while an adverse effect was observed under dark conditions. More detailed phytochemical analysis showed maximum total silymarin content (11.92 mg/g dry weight (DW)) when placed under continuous light + 1.0 mg/L melatonin. Individually, the level of silybins (A and B), silydianin, isolsilychristin and silychristin was found highest under continuous light. Anti-inflammatory activities were also studied and highest percent inhibition was recorded against 15-lipoxygenase (15-LOX) for cultures cultivated under continuous light (42.33%). The current study helps us to better understand the influence of melatonin and different light regimes on silymarin production as well as antioxidant and anti-inflammatory activities in callus extracts.
(L.) Gaertn. 是一种著名的药用植物，主要用于肝脏保护。光是植物中几种生理过程和次生代谢物生物合成的重要影响因素。在此，研究了在不同光照条件下，添加褪黑素对 的离体生物合成潜力。在光周期下，1.0mg/Lα-萘乙酸和 0.5mg/L6-苄基氨基嘌呤组合下，愈伤组织的最佳诱导率。连续光照与褪黑素处理增加了总类黄酮含量（TFC）、总酚含量（TPC）和抗氧化能力，其次是光周期和黑暗处理。在连续光照和光周期下，较高水平的褪黑素对生物量积累有协同作用，而在黑暗条件下则有不利影响。更详细的植物化学分析表明，在连续光照+1.0mg/L 褪黑素下，总水飞蓟素含量（11.92mg/g 干重（DW））达到最高。单独来看，在连续光照下，水飞蓟宾（A 和 B）、水飞蓟宁、异水飞蓟宾和水飞蓟宾的含量最高。还研究了抗炎活性，在连续光照下培养的细胞提取物对 15-脂氧合酶（15-LOX）的抑制率最高（42.33%）。本研究有助于更好地了解褪黑素和不同光照条件对水飞蓟素产生以及抗氧化和抗炎活性的影响。
