Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Videnska 1083, 142 20 Prague 4, Czech Republic.
Institute of Microbiology, Academy of Sciences of the Czech Republic, Videnska 1083, 142 20 Prague 4, Czech Republic.
Int J Mol Sci. 2019 Mar 29;20(7):1581. doi: 10.3390/ijms20071581.
Commensal microbiota contribute to gut homeostasis by inducing transcription of mucosal genes. Analysis of the impact of various microbiota on intestinal tissue provides an important insight into the function of this organ. We used cDNA microarrays to determine the gene expression signature of mucosa isolated from the small intestine and colon of germ-free (GF) mice and animals monoassociated with two strains. The results were compared to the expression data obtained in conventionally reared (CR) mice. In addition, we analyzed gene expression in colon organoids derived from CR, GF, and monoassociated animals. The analysis revealed that the complete absence of intestinal microbiota mainly affected the mucosal immune system, which was not restored upon monoassociation. The most important expression changes observed in the colon mucosa indicated alterations in adipose tissue and lipid metabolism. In the comparison of differentially expressed genes in the mucosa or organoids obtained from GF and CR mice, only six genes were common for both types of samples. The results show that the increased expression of the angiopoietin-like 4 () gene encoding a secreted regulator of lipid metabolism indicates the GF status.
共生微生物通过诱导黏膜基因的转录来维持肠道内环境稳态。分析各种微生物群落在肠道组织中的影响，为该器官的功能提供了重要的见解。我们使用 cDNA 微阵列来确定无菌（GF）小鼠和单定植两种菌株的小肠和结肠黏膜分离物的基因表达特征。将结果与常规饲养（CR）小鼠获得的表达数据进行比较。此外，我们还分析了源自 CR、GF 和单定植动物的结肠类器官中的基因表达。分析表明，肠道微生物群的完全缺失主要影响黏膜免疫系统，而单定植并不能使其恢复。在对来自 GF 和 CR 小鼠的结肠黏膜中差异表达基因的分析中，只有 6 个基因在两种类型的样本中都有表达。结果表明，编码脂质代谢调节剂的血管生成素样 4（）基因的表达增加表明处于 GF 状态。
