梓醇内酯对 LPS 诱导的 RAW264.7 细胞的抗炎作用。
Anti-Inflammatory Effects of Catalpalactone Isolated from in LPS-Induced RAW264.7 Cells.
机构信息
Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup 56212, Korea.
College of Pharmacy, Graduate School of Pharmaceutical Science, Ewha Womans University, Seoul 03760, Korea.
出版信息
Molecules. 2019 Mar 29;24(7):1236. doi: 10.3390/molecules24071236.
(Bignoniaceae) is widely distributed throughout Korea, China, and Japan. This study investigated the anti-inflammatory effects of catalpalactone isolated from in lipopolysaccharide (LPS)-induced RAW264.7 cells. Catalpalactone significantly inhibited nitric oxide (NO) production and inducible NO synthase (iNOS) expression in LPS-induced RAW264.7 cells. The levels of cytokines such as interleukin-6 and tumor necrosis factor-α were reduced under catalpalactone exposure in LPS-induced RAW264.7 cells. Additionally, catalpalactone suppressed signal transducer and activator of transcription 1 (STAT-1) protein expression and interferon-β (IFN-β) production. Treatment with catalpalactone prevented interferon regulatory factor 3 (IRF3) and nuclear factor-κB (NF-κB) activation. Taken together, these results suggest that the anti-inflammatory effects of catalpalactone are associated with the suppression of NO production and iNOS expression through the inhibition of IRF3, NF-κB, and IFN-β/STAT-1 activation.
梓属（Bignoniaceae）广泛分布于韩国、中国和日本。本研究探讨了从梓属中分离得到的梓醇内酯对脂多糖（LPS）诱导的 RAW264.7 细胞的抗炎作用。梓醇内酯显著抑制 LPS 诱导的 RAW264.7 细胞中一氧化氮（NO）的产生和诱导型一氧化氮合酶（iNOS）的表达。在 LPS 诱导的 RAW264.7 细胞中，梓醇内酯暴露后细胞因子如白细胞介素-6 和肿瘤坏死因子-α的水平降低。此外，梓醇内酯抑制信号转导和转录激活因子 1（STAT-1）蛋白表达和干扰素-β（IFN-β）的产生。梓醇内酯处理可防止干扰素调节因子 3（IRF3）和核因子-κB（NF-κB）的激活。综上所述，这些结果表明，梓醇内酯的抗炎作用与通过抑制 IRF3、NF-κB 和 IFN-β/STAT-1 激活来抑制 NO 产生和 iNOS 表达有关。
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