Key Laboratory of Plant Resource Conservation and Sustainable Utilization, Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.
Long Ping Branch, Graduate School of Hunan University, Changsha 410125, China.
Biomolecules. 2019 Mar 29;9(4):125. doi: 10.3390/biom9040125.
To prevent citrus decay caused by , 12 natural products were isolated from two endophytic fungi, in which cytosporone B was shown to have excellent bioactivity for control of with median effect concentration (EC) of 26.11 μg/mL and minimum inhibitory concentration (MIC) of 105 μg/mL, and also significantly reduced the decay of sugar orange during the in vivo trials. In addition, cytosporone B could alter the morphology of by causing distortion of the mycelia and loss of membrane integrity. Differentially expressed genes (DEGs) between cytosporone B-treated and -untreated samples were revealed by Illumina sequencing, including 3540 unigenes. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses showed that most DEGs were related to metabolic production and cell membrane. These findings suggest cytosporone B is a promising biological preservative to control citrus decay and reveal the action mechanism of cytosporone B in relation to the destruction of the fungal cell membrane at both morphological and molecular levels.
为了预防 引起的柑橘腐烂，从两种内生真菌中分离出 12 种天然产物，其中细胞松弛素 B 对 具有优异的生物活性，其半数效应浓度（EC）为 26.11μg/mL，最小抑菌浓度（MIC）为 105μg/mL，并且在体内试验中还显著降低了糖橙的腐烂率。此外，细胞松弛素 B 可以通过引起菌丝扭曲和膜完整性丧失来改变 的形态。通过 Illumina 测序揭示了细胞松弛素 B 处理和未处理样品之间的差异表达基因（DEGs），包括 3540 个基因。基因本体论（GO）和京都基因与基因组百科全书（KEGG）分析表明，大多数 DEGs 与代谢产物和细胞膜有关。这些发现表明细胞松弛素 B 是一种有前途的生物防腐剂，可以控制柑橘腐烂，并揭示了细胞松弛素 B 在破坏真菌细胞膜方面的形态和分子水平的作用机制。
