Odeleye Tinu, White William Lindsey, Lu Jun
School of Science, Faculty of Health and Environmental Sciences, Auckland University of Technology, Auckland 1010, New Zealand.
College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518071, China.
Biomedicines. 2019 Mar 30;7(2):25. doi: 10.3390/biomedicines7020025.
In this study, we examined the cytotoxic effects of four fractions from three species of New Zealand (NZ) surf clam on four common organ cancer cells. In most cases, a dose- and time-dependent inhibition on the proliferation of the cancer cells was observed. This was most significant in WiDr (colon) cells, where the percentages of viability reduced to as low as 6%, 5%, and 17% (at 1000 µg 72 h) by extracts from Diamond shell, Storm shell, and Tua tua species, respectively. A549 (lung) cells were the least susceptible to the treatment, with viability percentages at 82%, 15%, and 45%, under the same conditions. Induction of caspase-dependent apoptosis and alterations to the cell cycle further supported the observed morphological analysis. The ethanol, petroleum ether, and ethyl acetate fractions of NZ surf clam, rich in lipids and proteins, were more potent than their water-based counterpart. This is the first demonstration where extracts from NZ surf clams show the ability to inhibit the growth and proliferation of cancer cell lines. We suggest that NZ surf clam extracts have the potential to be further studied and developed as candidates for cancer supplementary management/treatment.
在本研究中，我们检测了三种新西兰 surf 蛤的四个组分对四种常见器官癌细胞的细胞毒性作用。在大多数情况下，观察到对癌细胞增殖的剂量和时间依赖性抑制。这在 WiDr（结肠）细胞中最为显著，在该细胞中，分别用钻石壳、风暴壳和 Tua tua 蛤的提取物处理后，在 1000 µg 72 小时时，细胞活力百分比分别降至低至 6%、5%和 17%。A549（肺）细胞对该处理最不敏感，在相同条件下，细胞活力百分比分别为 82%、15%和 45%。半胱天冬酶依赖性凋亡的诱导和细胞周期的改变进一步支持了所观察到的形态学分析。富含脂质和蛋白质的新西兰 surf 蛤的乙醇、石油醚和乙酸乙酯组分比其水相组分更具效力。这是首次证明新西兰 surf 蛤提取物具有抑制癌细胞系生长和增殖的能力。我们建议，新西兰 surf 蛤提取物有潜力作为癌症辅助管理/治疗的候选物进行进一步研究和开发。
