Sunil Kumar D, Janakiram P, Murali Krishna Kumar M, Krishna Geetha G
Department of Marine Living Resources, Andhra University, Visakhapatnam, Andhra Pradesh, 530003, India.
AU College of Pharmaceutical Sciences, Andhra University, Visakhapatnam, Andhra Pradesh, 530003, India.
World J Microbiol Biotechnol. 2017 Nov 3;33(11):207. doi: 10.1007/s11274-017-2340-4.
The crude extract isolated from the visceral mass of Anadara granosa, an intertidal bivalve mollusc was tested for inhibitory activity against pathogenic bacteria of the shrimp and fish viz. Vibrio harveyi and Staphylococcus aureus respectively by agar well diffusion and contact bioautography methods. Maximum inhibitory activity was shown against V. harveyi by methanol and chloroform (9:1) extract. Twelve fractions (1-12) could be separated from the crude extract through column chromatography. Five out of twelve fractions (7, 8, 9, 10, and 11) showed antibacterial activity and they were further run on column chromatography for purity. The fraction no. 9 showed highest antibacterial activity among the five and was subjected to NMR for the proton, C and H-H correlation, IR and mass spectral analysis for structural elucidation. Structure of the compound isolated from fraction no: 9 was determined as 1-(((2Z, 4Z)-dodeca-2,4-dienoyl)oxy)-3-hydroxypropan-2-yl tetradecanoate.
从潮间带双壳贝类软体动物颗粒蚶的内脏团中分离得到的粗提物，分别通过琼脂孔扩散法和接触生物自显影法，对虾类和鱼类的病原菌即哈维氏弧菌和金黄色葡萄球菌进行了抑制活性测试。甲醇和氯仿（9:1）提取物对哈维氏弧菌显示出最大抑制活性。通过柱色谱法可从粗提物中分离出12个馏分（1 - 12）。12个馏分中的5个（7、8、9、10和11）显示出抗菌活性，它们进一步进行柱色谱分离以提高纯度。馏分9在这5个中显示出最高的抗菌活性，并对其进行了核磁共振氢谱、碳谱和氢氢相关谱分析、红外光谱和质谱分析以阐明结构。从馏分9中分离得到的化合物结构确定为1 - ((((2Z, 4Z)-十二碳-2,4-二烯酰基)氧基)-3-羟基丙-2-基十四烷酸酯。
