Zhou Jun-Xian, Braun Markus Santhosh, Wetterauer Pille, Wetterauer Bernhard, Wink Michael
Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, Im Neuenheimer Feld 364, 69120 Heidelberg, Germany.
Medicines (Basel). 2019 Mar 30;6(2):43. doi: 10.3390/medicines6020043.
The phytochemical composition, antioxidant, cytotoxic, and antimicrobial activities of a methanol extract from L. (Ge), a 50% ethanol (in water) extract from Pall. (Pe), and a 96% ethanol extract from (Thunb.) Lindl. (Ue) were investigated. The phytochemical profiles of the extracts were analyzed by LC-MS/MS. Antioxidant activity was evaluated by scavenging 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radicals and reducing ferric complexes, and the total phenolic content was tested with the Folin⁻Ciocalteu method. Cytotoxicity was determined with a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay in murine macrophage RAW 264.7 cells. Antimicrobial activity of the three plant extracts was investigated against six bacterial strains with the broth microdilution method. Only Pe showed high antioxidant activities compared to the positive controls ascorbic acid and (-)-epigallocatechin gallate (EGCG) in DPPH assay; and generally the antioxidant activity order was ascorbic acid or EGCG > Pe > Ue > Ge. The three plant extracts did not show strong cytotoxicity against RAW 264.7 cells after 24 h treatment with IC values above 60.53 ± 4.03 μg/mL. Ue was not toxic against the six tested bacterial strains, with minimal inhibitory concentration (MIC) values above 5 mg/mL. Ge showed medium antibacterial activity against , , , , and with MICs between 0.31 and 1.25 mg/mL. Pe inhibited the growth of , , and at a MIC of 0.08 mg/mL. The three extracts were low-cytotoxic, but Pe exhibited effective DPPH radical scavenging ability and good antibacterial activity; Ue did not show antioxidant or antibacterial activity; Ge had no antioxidant potential, but medium antibacterial ability against five bacteria strains. Pe and Ge could be further studied for their potential to be developed as antioxidant or antibacterial candidates.
对光果甘草（L. (Ge)）的甲醇提取物、粗茎婆罗门参（Pall. (Pe)）的50%乙醇（水溶液）提取物和郁金香（(Thunb.) Lindl. (Ue)）的96%乙醇提取物的植物化学成分、抗氧化、细胞毒性和抗菌活性进行了研究。通过液相色谱-串联质谱（LC-MS/MS）分析提取物的植物化学图谱。通过清除2,2-二苯基-1-苦基肼（DPPH）和2,2'-联氮-双-（3-乙基苯并噻唑啉-6-磺酸）（ABTS）自由基以及还原铁络合物来评估抗氧化活性，并用福林-酚（Folin⁻Ciocalteu）法检测总酚含量。用3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）法在小鼠巨噬细胞RAW 264.7细胞中测定细胞毒性。用肉汤微量稀释法研究三种植物提取物对六种细菌菌株的抗菌活性。在DPPH测定中，与阳性对照抗坏血酸和（-）-表没食子儿茶素没食子酸酯（EGCG）相比，只有Pe表现出高抗氧化活性；一般抗氧化活性顺序为抗坏血酸或EGCG > Pe > Ue > Ge。用IC值高于60.53±4.03μg/mL处理24小时后，三种植物提取物对RAW 264.7细胞未显示出强细胞毒性。Ue对六种受试细菌菌株无毒，最小抑菌浓度（MIC）值高于5mg/mL。Ge对金黄色葡萄球菌、枯草芽孢杆菌、大肠杆菌、铜绿假单胞菌和白色念珠菌显示出中等抗菌活性，MIC在0.31至1.25mg/mL之间。Pe在MIC为0.08mg/mL时抑制金黄色葡萄球菌、枯草芽孢杆菌和大肠杆菌的生长。三种提取物细胞毒性低，但Pe表现出有效的DPPH自由基清除能力和良好的抗菌活性；Ue未显示抗氧化或抗菌活性；Ge没有抗氧化潜力，但对五种细菌菌株有中等抗菌能力。Pe和Ge作为抗氧化或抗菌候选物的潜力可进一步研究。
