Ye Zi, Dai Jia-Rong, Zhang Cheng-Gang, Lu Ye, Wu Lei-Lei, Gong Amy G W, Xu Hong, Tsim Karl W K, Wang Zheng-Tao
The Ministry of Education Key Laboratory for Standardization of Chinese Medicines, The State Administration of Traditional Chinese Medicine Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Shanghai R&D Center for Standardization of Chinese Medicines, Shanghai 201203, China.
Evid Based Complement Alternat Med. 2017;2017:8647212. doi: 10.1155/2017/8647212. Epub 2017 Jul 10.
The stems of Kimura et Migo (Dendrobii Officinalis Caulis) have a high medicinal value as a traditional Chinese medicine (TCM). Because of the limited supply, is a high priced TCM, and therefore adulterants are commonly found in the herbal market. The dried stems of a closely related species, Paxt., are commonly used as the substitute; however, there is no effective method to distinguish the two species. Here, a high performance liquid chromatography (HPLC) method was successfully developed and applied to differentiate and by comparing the chromatograms according to the characteristic peaks. A HPLC coupled with electrospray ionization multistage mass spectrometry (HPLC-ESI-MS) method was further applied for structural elucidation of 15 flavonoids, 5 phenolic acids, and 1 lignan in . Among these flavonoids, 4 flavonoid C-glycosides were firstly reported in , and violanthin and isoviolanthin were identified to be specific for compared with . Then, two representative components were used as chemical markers. A rapid and reliable high performance thin layer chromatography (HPTLC) method was applied in distinguishing from . The results of this work have demonstrated that these developed analytical methods can be used to discriminate and effectively and conveniently.
金钗石斛（Dendrobii Officinalis Caulis）的茎作为一种传统中药具有很高的药用价值。由于其供应有限，是一种价格高昂的中药，因此在草药市场上常见掺假情况。近缘种束花石斛（Dendrobium wardianum Paxt.）的干燥茎常被用作替代品；然而，尚无有效的方法来区分这两个物种。在此，成功开发了一种高效液相色谱（HPLC）方法，并通过根据特征峰比较色谱图来应用于区分金钗石斛和束花石斛。进一步应用HPLC与电喷雾电离多级质谱联用（HPLC-ESI-MS）方法对金钗石斛中的15种黄酮类化合物、5种酚酸和1种木脂素进行结构解析。在这些黄酮类化合物中，4种黄酮碳苷首次在金钗石斛中被报道，与束花石斛相比，紫堇黄质和异紫堇黄质被鉴定为金钗石斛特有的成分。然后，将两种代表性成分用作化学标志物。应用一种快速可靠的高效薄层色谱（HPTLC）方法来区分金钗石斛和束花石斛。这项工作的结果表明，这些开发的分析方法可用于有效且方便地鉴别金钗石斛和束花石斛。
