Cohen M D, Wei C I, Tan H, Kao K J
J Toxicol Environ Health. 1986;19(2):279-98. doi: 10.1080/15287398609530927.
Female B6C3F1 mice were exposed to ammonium metavanadate (NH4VO3) by intraperitoneal injection every 3 d at 2.5, 5.0, or 10 mg V/kg for 3, 6, or 9 w and were then assayed for alterations in immunoresponsiveness. Resistance to Escherichia coli endotoxin lethality increased in a dose-dependent manner up to 6 w of exposure, while resistance to viable gram-positive Listeria lethality was depressed in a dose-dependent manner. Comparison of LD20 values indicated a 250-fold decrease in resistance to Listeria at the lowest vanadium exposure and a 40% increase in resistance to endotoxin after the highest vanadium exposure. Peritoneal macrophage phagocytic capacities were decreased in a dose-dependent manner, but viabilities remained unaffected. Rosetting capacity of splenic lymphocytes was increased following vanadium exposure. Liver and splenic enlargement was observed, and examination of splenic tissue indicated enhanced formation of megakaryocytes and red blood cell precursors. Subchronic exposure to vanadium may thus disrupt the normal function of the immune system.
雌性B6C3F1小鼠每隔3天腹腔注射偏钒酸铵（NH4VO3），剂量分别为2.5、5.0或10毫克钒/千克，持续3、6或9周，然后检测免疫反应性的变化。在暴露长达6周的时间里，对大肠杆菌内毒素致死的抵抗力呈剂量依赖性增加，而对活的革兰氏阳性李斯特菌致死的抵抗力则呈剂量依赖性降低。对半数致死剂量（LD20）值的比较表明，在最低钒暴露水平下，对李斯特菌的抵抗力下降了250倍，而在最高钒暴露后，对内毒素的抵抗力增加了40%。腹腔巨噬细胞的吞噬能力呈剂量依赖性降低，但活力不受影响。钒暴露后脾淋巴细胞的玫瑰花结形成能力增强。观察到肝脏和脾脏肿大，对脾脏组织的检查表明巨核细胞和红细胞前体的形成增加。因此，亚慢性钒暴露可能会扰乱免疫系统的正常功能。
