Vaddi K, Wei C I
Food Science and Human Nutrition Department, University of Florida, Gainesville 32611-0163.
J Toxicol Environ Health. 1991 May;33(1):65-78. doi: 10.1080/15287399109531506.
Female B6C3F1 mice were injected intraperitoneally with ammonium metavanadate (2.5 or 10 mg V/kg), ammonium chloride, or sodium phosphate buffer (0.1 M, pH 7.2) every 3 d for 6 wk. Resident peritoneal macrophage (PEM) cytolysates were prepared and assayed for intracellular enzyme activities of beta-glucuronidase, N-acetyl-beta-D-glucosaminidase, acid phosphatase, and lysozyme, to investigate possible reasons for the depressive effect of ammonium metavanadate on the intracellular killing of Listeria monocytogenes by murine PEM. Acid phosphatase activity per 10(6) cells for the 2.5 and 10 mg V/kg groups was depressed by 22.8 and 44.7%, respectively, when compared to phosphate buffer controls. No significant effect by vanadium treatment was observed with regard to the other three enzymes. Kinetic studies (in vitro) on the effect of ammonium metavanadate (5, 10, 15, and 20 mM) on the above enzymes showed similar patterns of effect by vanadium. Lineweaver-Burk analysis of acid phosphatase indicated linear noncompetitive inhibition by vanadium with a Kj of 14.8 mM. NH4Cl and 10 mg V/kg treatments also enhanced extracellular secretion of beta-glucuronidase and lysozyme from PEM, which could be attributed to the presence of ammonium ion. The decrease in acid phosphatase activity might contribute, in part through its interference in the phosphorylation/dephosphorylation, to the diminished intracellular killing ability of PEM.
将雌性B6C3F1小鼠每3天腹腔注射一次偏钒酸铵（2.5或10 mg钒/千克）、氯化铵或磷酸钠缓冲液（0.1 M，pH 7.2），持续6周。制备驻留腹膜巨噬细胞（PEM）细胞裂解物，并检测β-葡萄糖醛酸酶、N-乙酰-β-D-氨基葡萄糖苷酶、酸性磷酸酶和溶菌酶的细胞内酶活性，以研究偏钒酸铵对小鼠PEM细胞内杀灭单核细胞增生李斯特菌的抑制作用的可能原因。与磷酸盐缓冲液对照组相比，2.5和10 mg钒/千克组每10⁶个细胞的酸性磷酸酶活性分别降低了22.8%和44.7%。钒处理对其他三种酶未观察到显著影响。关于偏钒酸铵（5、10、15和20 mM）对上述酶的影响的动力学研究（体外）显示钒具有相似的作用模式。酸性磷酸酶的Lineweaver-Burk分析表明钒呈线性非竞争性抑制，Kj为14.8 mM。氯化铵和10 mg钒/千克处理也增强了PEM细胞外β-葡萄糖醛酸酶和溶菌酶的分泌，这可能归因于铵离子的存在。酸性磷酸酶活性的降低可能部分通过干扰磷酸化/去磷酸化，导致PEM细胞内杀伤能力减弱。
