Chilton F H, Murphy R C
Prostaglandins Leukot Med. 1986 Aug;23(2-3):141-8. doi: 10.1016/0262-1746(86)90177-0.
Neutrophils were labeled with high specific activity, [3H]-arachidonate and separated into their arachidonate-containing phosphoglycerides using a slight modification of the normal phase and reverse phase HPLC described by Patton et al. The individual molecular species of PC, PE and PI containing arachidonate were characterized by fast atom bombardment/mass spectrometry techniques. Eight major arachidonate-containing molecular species of PC, six major arachidonate-containing molecular species of PE and two major arachidonate-containing molecular species of PI could be found. With the structures of the phosphoglycerides eluting from the reverse phase HPLC column known, general trends in the HPLC elution behavior of 1-0-alkyl, 1-alk-1-enyl and 1-acyl-2-arachidonoyl phosphoglycerides could be observed.
用高比活度的[3H] - 花生四烯酸标记中性粒细胞，并使用对Patton等人描述的正相和反相高效液相色谱法（HPLC）稍作修改的方法，将其分离成含花生四烯酸的磷酸甘油酯。通过快原子轰击/质谱技术对含花生四烯酸的PC、PE和PI的各个分子种类进行了表征。可以发现PC的八种主要含花生四烯酸分子种类、PE的六种主要含花生四烯酸分子种类和PI的两种主要含花生四烯酸分子种类。由于已知从反相HPLC柱洗脱的磷酸甘油酯的结构，因此可以观察到1 - O - 烷基、1 - 烯 - 1 - 烯基和1 - 酰基 - 2 - 花生四烯酰基磷酸甘油酯在HPLC洗脱行为中的一般趋势。
