趋化肽诱导人多形核白细胞中花生四烯酸的动员。
Chemotactic peptide-induced arachidonic acid mobilization in human polymorphonuclear leukocytes.
作者信息
Galbraith G M
机构信息
Department of Microbiology and Immunology, Medical University of South Carolina, Charleston 29425-2230.
出版信息
Am J Pathol. 1988 Nov;133(2):347-54.
Human polymorphonuclear leukocytes prelabeled with tritiated arachidonic acid liberated radiolabeled products when exposed to the chemotactic peptide fMet-Leu-Phe. The effect was enhanced in the presence of phorbol-12-myristate 13-acetate or 1-oleoyl-2-acetyl glycerol; these agents activate phospholipid- and Ca2+-dependent protein kinase C. In contrast, arachidonic acid mobilization was suppressed by two compounds known to inhibit protein kinase C activity: polymyxin B and 1-(5-isoquinolinylsulfonyl)-2-methylpiperazine. These results suggest that protein kinase C was involved in arachidonic acid mobilization in leukocytes stimulated with chemotactic peptide.
预先用氚标记的花生四烯酸标记的人多形核白细胞在暴露于趋化肽fMet-Leu-Phe时会释放出放射性标记产物。在佛波醇-12-肉豆蔻酸酯13-乙酸酯或1-油酰基-2-乙酰甘油存在的情况下，这种效应会增强；这些试剂可激活磷脂和Ca2+依赖性蛋白激酶C。相比之下，花生四烯酸的动员受到两种已知可抑制蛋白激酶C活性的化合物的抑制：多粘菌素B和1-(5-异喹啉磺酰基)-2-甲基哌嗪。这些结果表明，蛋白激酶C参与了趋化肽刺激的白细胞中花生四烯酸的动员。
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