Eastern Hepatobiliary Surgery Hospital (EHBH), Second Military Medical University, Shanghai City 200438, China.
The Seventh People's Hospital of Shanghai, Shanghai City 200137, China.
BMB Rep. 2019 May;52(5):312-317. doi: 10.5483/BMBRep.2019.52.5.155.
Hepatocellular carcinoma (HCC) is the leading cause of cancer-related mortality worldwide. MiR-371 has recently emerged as an important regulator in tumorigenesis, and may serve as a biomarker for malignant tumors. We transfected miR-371 or its inhibitor in two human HCC cell lines, then used 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, soft agar colony formation, and transwell migration assays to evaluate the effects on cell proliferation, migration, and invasion. We found that miR-371 was positively correlated with HCC metastasis and poor prognosis in the inflicted patients, and the high expression of miR-371 was promoted, whereas a low level of miR-371 depressed cell proliferation and invasion. We found PTEN to be a direct target of miR-371. The overexpression or knockdown of PTEN exhibited the opposite effects from those of miR-371 on cell proliferation and migration. Our study demonstrates that miR-371 promotes proliferation and metastasis in HCC by targeting PTEN. [BMB Reports 2019; 52(5): 312-317].
肝细胞癌 (HCC) 是全球癌症相关死亡的主要原因。miR-371 最近成为肿瘤发生的重要调节剂，并且可以作为恶性肿瘤的生物标志物。我们转染了 miR-371 或其抑制剂在两种人 HCC 细胞系中，然后使用 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐、软琼脂集落形成和 Transwell 迁移实验来评估对细胞增殖、迁移和侵袭的影响。我们发现 miR-371 与受影响患者的 HCC 转移和不良预后呈正相关，miR-371 的高表达促进了细胞增殖和侵袭，而低水平的 miR-371 则抑制了细胞增殖和侵袭。我们发现 PTEN 是 miR-371 的直接靶标。PTEN 的过表达或敲低表现出与 miR-371 对细胞增殖和迁移的相反作用。我们的研究表明，miR-371 通过靶向 PTEN 促进 HCC 的增殖和转移。[BMB 报告 2019;52(5):312-317]。
