Dietel M, Arps H, Rohlff A, Bodecker R, Niendorf A
Virchows Arch A Pathol Anat Histopathol. 1986;409(6):829-36. doi: 10.1007/BF00710767.
Scanning-DNA cytophotometry was applied to Feulgen stained sections of 22 borderline tumors of the ovary (BOT). The DNA content was related to conventional histology. In 11 cases clinical follow up for more than 5 years was available. The DNA measurements disclosed two subgroups in the group of BOT. One showed a nuclear DNA content not exceeding tetraploidy (4c) indicating proliferative activity without malignant change and a second one exhibited DNA values higher than 4c indicating malignant transformation. Correlation of histological evaluation with the DNA content revealed a good agreement in 15 cases. However, a discrepancy was found in 7 cases: either the histological evaluation aroused suspicion for malignant potential but the histogram showed DNA values not higher than 4c (n = 4), or histology showed well differentiated lesions with an atypical histogram (n = 3). Clinical monitoring revealed no recurrence or tumor spread in all but one case of the group of lesions with DNA values up to 4c, whereas in the group with atypical DNA histograms (DNA values greater than 4c) relapse appeared in 6 out of 7 cases. The results suggest that DNA analysis has prognostic significance for BOT.
对22例卵巢交界性肿瘤（BOT）的福尔根染色切片进行扫描DNA细胞光度测定。DNA含量与传统组织学相关。11例患者有超过5年的临床随访资料。DNA测量结果显示BOT组可分为两个亚组。一组显示核DNA含量不超过四倍体（4c），表明具有增殖活性但无恶性改变；另一组显示DNA值高于4c，表明发生了恶性转化。组织学评估与DNA含量的相关性在15例中显示出良好的一致性。然而，在7例中发现了差异：要么组织学评估引起对恶性潜能的怀疑，但直方图显示DNA值不高于4c（n = 4），要么组织学显示为分化良好的病变，但直方图不典型（n = 3）。临床监测显示，除1例DNA值高达4c的病变组外，其余病例均无复发或肿瘤扩散，而在DNA直方图不典型（DNA值大于4c）的组中，7例中有6例出现复发。结果表明，DNA分析对BOT具有预后意义。
