Fosså S D, Thorud E, Vaage S, Shoaib M C
Acta Pathol Microbiol Immunol Scand A. 1983 Jul;91(4):235-43.
In 18 biopsy specimens from human breast carcinoma a comparison was made between DNA measurements obtained by microspectrophotometry (MSP) of Feulgen-stained nuclei in imprints and flow cytometry (FCM) of nuclei stained with ethidium bromide. For each specimen FCM was performed both with ethanol-fixed cells and unfixed cells. In addition, single cell suspensions were made from other 11 fresh mammary cancer biopsies. Parts of these suspensions were analysed both by MSP (Feulgen-stained smears) and FCM (ethanol-fixed, mitramycin-stained cells). The MSP histograms show selected tumour cells and tumour-like cells. This explains the higher proportion of cells with DNA content above the 2 c level. A good agreement was found between the results obtained by MSP and FCM with regard to the ploidy of the DNA stemline(s). FCM of fixed cells (multiple-step procedure) yielded a slightly lower proportion of diploid cells than FCM of the unfixed cells (one-step procedure), probably owing to loss of small cells during the different preparation steps. It is concluded that the results from DNA-histograms obtained from MSP and FCM can be compared as to DNA-stemline ploidy of the cell population but not as to the proportion of cells with non-diploid DNA-content.
对取自人乳腺癌的18份活检标本进行了比较，比较了通过对印片中经福尔根染色的细胞核进行显微分光光度测定法（MSP）和对经溴化乙锭染色的细胞核进行流式细胞术（FCM）所获得的DNA测量结果。对于每份标本，分别对乙醇固定细胞和未固定细胞进行了流式细胞术检测。此外，还从另外11份新鲜乳腺癌活检标本制备了单细胞悬液。这些悬液的一部分分别通过显微分光光度测定法（福尔根染色涂片）和流式细胞术（乙醇固定、放线菌素D染色的细胞）进行分析。显微分光光度测定法的直方图显示了选定的肿瘤细胞和肿瘤样细胞。这就解释了DNA含量高于2c水平的细胞比例较高的原因。在DNA主干线的倍性方面，显微分光光度测定法和流式细胞术所获得的结果之间发现有良好的一致性。固定细胞的流式细胞术（多步骤程序）所得到的二倍体细胞比例略低于未固定细胞的流式细胞术（单步骤程序），这可能是由于在不同的制备步骤中小细胞丢失所致。得出的结论是，就细胞群体的DNA主干线倍性而言，显微分光光度测定法和流式细胞术所获得的DNA直方图结果可以进行比较，但就非二倍体DNA含量的细胞比例而言则不能进行比较。
