Friedlander M L, Taylor I W, Russell P, Musgrove E A, Hedley D H, Tattersall M H
Int J Gynecol Pathol. 1983;2(1):55-63. doi: 10.1097/00004347-198301000-00005.
The cellular DNA content of 50 ovarian common epithelial carcinomas was determined by flow cytometry, and tumours were classified as being either diploid or aneuploid. A significant association between tumour stage and ploidy was demonstrated, with all diploid tumours being of an early stage (p less than 0.001). Forty percent of early-stage tumours (FIGO stages I and II) and all late-stage tumours (FIGO stages III and IV) were aneuploid. This heterogeneity with respect to DNA content among tumours of a similar stage may allow the identification of neoplasms with a different natural history. The proportion of S-phase cells determined by flow cytometry is a measure of cellular proliferation and may also be of prognostic significance. Diploid tumours had a median S phase of 9.8% (2.4-14.1%), while aneuploid tumours had a significantly higher S phase of 19.6% (7-24.7%; p less than 0.05). In this study there was no relationship between histological grading of invasive carcinomas and ploidy, but in view of the relatively small numbers and limited follow-up, it was not possible to perform a multivariate analysis of all known prognostic factors in ovarian cancer. Our results suggest that ploidy reflects tumour behaviour, but prolonged follow-up and increased patient accrual is necessary to assess whether the flow cytometric analysis of DNA content will provide clinically important information in ovarian cancer.
采用流式细胞术测定50例卵巢常见上皮性癌的细胞DNA含量，并将肿瘤分为二倍体或非整倍体。结果显示肿瘤分期与倍体之间存在显著关联，所有二倍体肿瘤均为早期（p<0.001）。40%的早期肿瘤（FIGO Ⅰ期和Ⅱ期）及所有晚期肿瘤（FIGO Ⅲ期和Ⅳ期）为非整倍体。相似分期肿瘤间DNA含量的这种异质性可能有助于识别具有不同自然病程的肿瘤。通过流式细胞术测定的S期细胞比例是细胞增殖的一个指标，也可能具有预后意义。二倍体肿瘤的S期中位数为9.8%（2.4 - 14.1%），而非整倍体肿瘤的S期显著更高，为19.6%（7 - 24.7%；p<0.05）。在本研究中，浸润性癌的组织学分级与倍体之间无相关性，但鉴于样本数量相对较少且随访有限，无法对卵巢癌所有已知的预后因素进行多因素分析。我们的结果提示倍体反映肿瘤行为，但需要延长随访时间并增加患者入组数量，以评估DNA含量的流式细胞术分析是否能为卵巢癌提供具有临床重要性的信息。
