Paytuvi-Gallart Andreu, Sanseverino Walter, Aiese Cigliano Riccardo
Sequentia Biotech SL, Carrer Comte d'Urgell 240, Barcelona, Spain.
Methods Mol Biol. 2019;1933:397-414. doi: 10.1007/978-1-4939-9045-0_25.
Experimentally validated plant lncRNAs have been shown to regulate important agronomic traits such as phosphate starvation response, flowering time, and interaction with symbiotic organisms, making them of great interest in plant biology and in breeding. We developed a pipeline to annotate lncRNAs and applied it to 37 plant species and 6 algae, resulting in the annotation of more than 120,000 lncRNAs. To facilitate the study of lncRNAs for the plant research community, the information gathered is organized in the Green Non-Coding Database (GreeNC, http://greenc.sciencedesigners.com/) . This chapter contains a detailed explanation of the content of GreeNC and how to access both programmatically and with a web browser.
实验验证的植物长链非编码RNA（lncRNA）已被证明可调控重要农艺性状，如磷饥饿反应、开花时间以及与共生生物的相互作用，这使得它们在植物生物学和育种领域备受关注。我们开发了一个用于注释lncRNA的流程，并将其应用于37种植物和6种藻类，结果注释了超过120,000个lncRNA。为便于植物研究群体研究lncRNA，所收集的信息整理在绿色非编码数据库（GreeNC，http://greenc.sciencedesigners.com/）中。本章详细解释了GreeNC的内容以及如何通过编程方式和网络浏览器进行访问。
