Pinkney Holly R, Wright Brandon M, Diermeier Sarah D
Department of Biochemistry, University of Otago, Dunedin 9016, New Zealand.
Noncoding RNA. 2020 Dec 16;6(4):49. doi: 10.3390/ncrna6040049.
Long non-coding RNAs (lncRNAs) are a rapidly expanding field of research, with many new transcripts identified each year. However, only a small subset of lncRNAs has been characterized functionally thus far. To aid investigating the mechanisms of action by which new lncRNAs act, bioinformatic tools and databases are invaluable. Here, we review a selection of computational tools and databases for the in silico analysis of lncRNAs, including tissue-specific expression, protein coding potential, subcellular localization, structural conformation, and interaction partners. The assembled lncRNA toolkit is aimed primarily at experimental researchers as a useful starting point to guide wet-lab experiments, mainly containing multi-functional, user-friendly interfaces. With more and more new lncRNA analysis tools available, it will be essential to provide continuous updates and maintain the availability of key software in the future.
长链非编码RNA（lncRNAs）是一个迅速发展的研究领域，每年都有许多新的转录本被鉴定出来。然而，到目前为止，只有一小部分lncRNAs在功能上得到了表征。为了有助于研究新lncRNAs发挥作用的机制，生物信息学工具和数据库非常宝贵。在这里，我们回顾了一系列用于lncRNAs计算机分析的计算工具和数据库，包括组织特异性表达、蛋白质编码潜力、亚细胞定位、结构构象和相互作用伙伴。组装的lncRNA工具包主要面向实验研究人员，作为指导湿实验室实验的有用起点，主要包含多功能、用户友好的界面。随着越来越多新的lncRNA分析工具问世，未来持续更新并保持关键软件的可用性将至关重要。
