a Department of Genetic and Bioengineering, Faculty of Engineering and Natural Sciences , Gaziosmanpaşa University , Tokat , Turkey.
Artif Cells Nanomed Biotechnol. 2019 Dec;47(1):1404-1415. doi: 10.1080/21691401.2019.1596933.
Hypericum perforatum oil loaded electrospun polymeric wound dressing material was produced in order to be used in wound therapy. H. perforatum oil is known to have curative effect on wound-healing process. Wound dressing material was produced in two layers, upper layer of which was made of electrospun PCL nanofibres in order to maintain membrane integrity and mechanical strength, and bottom layer that is designed to be in contact with the wound was formed by electrospraying and electrospining of PEG/H. perforatum oil and PCL polymer solutions from opposite directions (concurrently). Methods such as FTIR, optical and electron microscopy, tensile tests, gas permeability tests, contact angle and swelling tests, in vitro release tests were utilized for material characterization. Encapsulation of H. perforatum oil in PEG capsules which were hold by PEG fibres among PCL fibres was confirmed. H. perforatum oil was released in controlled manner. Antimicrobial activity tests on Staphylococcus aureus and Escherichia coli revealed that H. perforatum content exhibited antimicrobial activity on both. Material was found to be biocompatible and suitable for use as wound dressing according to the results of in vitro tests, in which L929 mouse fibroblast cell line incubated with materials for investigation of biocompatibility (WST-1) and cell-material interactions (proliferation, apoptosis/necrosis).
为了应用于伤口治疗，制备了贯叶连翘油负载的电纺聚合物创伤敷料材料。贯叶连翘油具有促进伤口愈合的功效。创伤敷料材料由上下两层组成，上层由电纺的 PCL 纳米纤维制成，以保持膜的完整性和机械强度，而与伤口接触的底层则由 PEG/贯叶连翘油和 PCL 聚合物溶液通过相反的方向（同时）静电纺丝和静电喷涂形成。采用傅里叶变换红外光谱（FTIR）、光学显微镜和电子显微镜、拉伸试验、透气性能测试、接触角和溶胀试验、体外释放试验等方法对材料进行了表征。证实了贯叶连翘油被包封在 PEG 胶囊中，PEG 胶囊被 PEG 纤维固定在 PCL 纤维之间。贯叶连翘油以控制的方式释放。对金黄色葡萄球菌和大肠杆菌的抗菌活性测试表明，贯叶连翘油含量对两者均具有抗菌活性。根据体外试验的结果，该材料被发现是生物相容的，适合用作创伤敷料，其中 L929 小鼠成纤维细胞系与用于研究生物相容性（WST-1）和细胞-材料相互作用（增殖、凋亡/坏死）的材料一起孵育。
