Chemical Engineering Department, Engineering Faculty, Mersin University, Mersin, Turkey.
Chemical Engineering Department, Engineering Faculty, Mersin University, Mersin, Turkey.
Int J Biol Macromol. 2020 Oct 15;161:1581-1590. doi: 10.1016/j.ijbiomac.2020.08.056. Epub 2020 Aug 7.
In this study, a herbal infused oil (Hypericum perforatum, HP) incorporated chitosan (CS) cryogel as a wound dressing material was produced in order to be used in wound healing process. The main strategy is to combine the traditional perspective of using medicinal oils with polymeric scaffolds manufactured by an engineering approach to fabricate a potential tissue engineering product that provides both new tissue formation and wound healing. The scaffolds manufactured by cryogelation were soft, spongy, highly porous, physically stable, elastic and could be easily cut in any desired shape. Physicochemical, mechanical and morphological analyzes were used to characterize the produced cryogels. Young modulus of the plain chitosan cryogel was about 21 kPa whereas it increased with increasing HP oil content and became 61 kPa for 20% HP oil ratio. Further, the antimicrobial studies, antioxidant and DNA cleavage effects were investigated. Samples including the highest ratio of oil (CS4) showed the highest DPPH scavenging activity as 69.9%. In addition, 20% HP oil loaded chitosan cryogel demonstrated single strain DNA cleavage activitiy at 500 μg/mL concentration. Antimicrobial studies were applied against seven strains. The lowest activities were obtained against E. hirae and B. cereus, the highest against E. coli and L. pneumophila. This study concluded that the newly developed HP oil loaded chitosan cryogel scaffolds with unique antimicrobial and antioxidant properties are promising candidates to be used in tissue engineering applications as wound dressing for exudative and long-term healing wounds.
在这项研究中，我们制备了一种含草药油（贯叶连翘，HP）的壳聚糖（CS）水凝胶作为伤口敷料，用于伤口愈合过程。主要策略是将使用草药油的传统观点与通过工程方法制造的聚合支架相结合，以制造出一种既能提供新组织形成又能促进伤口愈合的潜在组织工程产品。通过冷冻凝胶法制备的支架柔软、有弹性、具有高度多孔性、物理稳定性好，且可轻松切割成任何所需的形状。我们使用物理化学、机械和形态分析来表征所制备的水凝胶。壳聚糖水凝胶的杨氏模量约为 21 kPa，而随着 HP 油含量的增加而增加，当 HP 油比例为 20%时达到 61 kPa。此外，我们还研究了其抗菌、抗氧化和 DNA 切割作用。包含最高油比（CS4）的样品表现出最高的 DPPH 清除活性，为 69.9%。此外，在 500 μg/mL 浓度下，负载 20%HP 油的壳聚糖水凝胶表现出单链 DNA 切割活性。我们对七种菌株进行了抗菌研究。对 E. hirae 和 B. cereus 的活性最低，对 E. coli 和 L. pneumophila 的活性最高。这项研究得出结论，具有独特的抗菌和抗氧化性能的新型 HP 油负载壳聚糖水凝胶支架是有前途的候选材料，可作为渗出性和长期愈合伤口的伤口敷料用于组织工程应用。
