Al-Tamimi Jameel, Al Omar Suliman Y, Aljuaimlani Ali, Mansour Lamjed
Department of Zoology, College of Science, King Saud University P. O. Box 2455, Riyadh 11451, Saudi Arabia.
Am J Transl Res. 2024 Nov 15;16(11):6646-6654. doi: 10.62347/PQKD5160. eCollection 2024.
Human leukocyte antigen-G () is linked to the development of human malignancies via immune escape mechanisms. The chief variations for were found in three prime untranslated regions (3'UTR). The current study aims to evaluate the distribution of (), , and polymorphisms with risk of acute lymphocytic leukemia (ALL) in Saudi Arabia. This case-control study analyzed 232 samples from 117 patients with ALL and 115 healthy controls (HCN) using the PCR-RFLP method. Associations between and ALL risk were analyzed using allele contrasts. The polymorphism results showed a reduced risk of ALL in the dominant model odds ratios (OR) = 0.34, 95% confidence interval (CI) = 0.12-0.98, = 0.041), stratified by age. However, those stratified by gender, showed decreased risk of ALL in all genetic inheritance models tested: codominant model versus (OR = 0.24, 95% CI = 0.06-0.99), versus (OR = 0.01, 95% CI = 0.00-0.12), = 0.0001), and dominant model versus (OR = 0.12, 95% CI = 0.03-0.47, = 0.000004), and the recessive model versus (OR = 0.03, 95% CI = 0.00-0.24, = 0.0001), log-additive (OR = 0.12, 95% CI = 0.04-0.35, = 0.0001). Conversely, the allele was not found in ALL or HCN, whereas the allele showed polymorphic frequencies that were not significant. In conclusion, the polymorphism significantly decreased the prevalence of ALL stratified by gender and age polymorphisms in the risk of ALL in the pediatric Saudi population.
人类白细胞抗原-G（HLA-G）通过免疫逃逸机制与人类恶性肿瘤的发生发展相关联。HLA-G的主要变异存在于3'非翻译区（3'UTR）。本研究旨在评估沙特阿拉伯人群中HLA-G（-1413G/A、+3187A/G和+3010C/G）基因多态性与急性淋巴细胞白血病（ALL）风险的关系。本病例对照研究采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法分析了117例ALL患者和115名健康对照（HCN）的232份样本。使用等位基因对比分析HLA-G与ALL风险之间的关联。HLA-G -1413G/A基因多态性结果显示，在按年龄分层的显性模型中（优势比[OR]=0.34，95%置信区间[CI]=0.12 - 0.98，P=0.041），ALL风险降低。然而，按性别分层时，在所有测试的遗传遗传模型中ALL风险均降低：共显性模型中A/A与G/G相比（OR = 0.24，95% CI = 0.06 - 0.99），G/A与G/G相比（OR = 0.01，95% CI = 0.00 - 0.12，P = 0.0001），显性模型中A/A与G/A + G/G相比（OR = 0.12，95% CI = 0.03 - 0.47，P = 0.000004），隐性模型中A/A与G/A相比（OR = 0.03，95% CI = 0.00 - 0.24，P = 0.0001），对数加性模型（OR = 0.12，95% CI = 0.04 - 0.35，P = 0.0001）。相反，在ALL患者或健康对照中均未发现+3187A/G等位基因，而+3010C/G等位基因的多态性频率无统计学意义。总之，在沙特儿科人群中，HLA-G -1413G/A基因多态性按性别和年龄分层时均显著降低了ALL的患病率，提示HLA-G基因多态性与ALL风险相关。
