Morenikeji Olanrewaju B, Akinyemi Mabel O, Wheto Mathew, Ogunshola Olawale J, Badejo Adebanjo A, Chineke Clifford A
Department of Animal Production and Health, Federal University of Technology, Akure 340252, Nigeria.
J Genet. 2019 Mar;98.
The expression of four genes involved in milk regulation and production in bovine milk and tissue samples profiled using quantitative PCR to identify differential gene expression. Our goal focussed on the differential mRNA expression of milk genes (, , and ) in milk samples and different tissues from four different breeds of ecologically adapted and geographically separated cattle species. The mRNA expression identified the four milk genes under studied most upregulated in mammary gland and milk samples as compared with other tissues. The expression of gene in the brain identified to have influenced the expression of and in the mammary and milk samples. Among the four genes, PRL had the highest mRNA expression (144.19-fold change) in Holstein followed by with 100.89-fold change, while the smallest relative expression for most genes in this study are in the range from 0.79 to 7.35-fold difference. White Fulani cattle was identified to have a higher expression for , and compared with Angus and Ndama cattle, while Holstein cattle is on top of the list on the basis of the gene expression and gene regulation for all the four genes in this study. Also, White Fulani and Holstein are in the same cluster based on their mRNA expression for milk genes. Our data showed the first evidence of the molecular identification of indigenous White Fulani cattle ofhaving potential for higher milk production.
使用定量PCR对牛奶和组织样本中参与牛奶调节和生产的四个基因进行表达分析，以鉴定差异基因表达。我们的目标聚焦于来自四个生态适应且地理隔离的牛品种的牛奶样本和不同组织中牛奶基因（、、和）的差异mRNA表达。mRNA表达表明，与其他组织相比，所研究的四个牛奶基因在乳腺和牛奶样本中上调程度最高。已确定大脑中基因的表达会影响乳腺和牛奶样本中基因和的表达。在这四个基因中，荷斯坦牛中PRL的mRNA表达最高（变化144.19倍），其次是变化100.89倍，而本研究中大多数基因的最小相对表达差异在0.79至7.35倍之间。与安格斯牛和恩达马牛相比，已确定白富拉尼牛中、和的表达较高，而在本研究中，基于所有四个基因的基因表达和基因调控，荷斯坦牛位居榜首。此外，基于牛奶基因的mRNA表达，白富拉尼牛和荷斯坦牛属于同一聚类。我们的数据首次证明了对本土白富拉尼牛进行分子鉴定，其具有更高产奶潜力。
