Zhang Cui, Xu Da, Chen Weina, Li Junliang, Gao Qinghua, Li Shijie
Department of Biochemistry and Molecular Biology, College of Life Sciences, Hebei Agriculture University, Baoding 071001, People's Republic of China.
J Genet. 2019 Mar;98.
Long noncoding RNAs (lncRNAs) are important regulators of biological processes, and regulate genomic imprinting in and/or to induce monoallelic expression with parent-origin-specific pattern. domain is one of the largest imprinted clusters in mammals, and maternally expressed noncoding RNAs of this region is related to the pluripotency of the embryonic stem cells. Previously, we sequenced the cDNA of two maternally expressed noncoding RNAs, and , and mapped a lncRNA () between the two genes in the cattle domain on chromosome 21. In this study, we identified , a novel long intergenic noncoding RNA (lincRNA), which was also located between and . We identified four variants of LINC24065 (, , and ) that were a result of alternative splicing from 18 exons. and showed tissue-specific expression patterns in adultbovine tissues, and and were detected in all eight analysed tissues (heart, liver, spleen, lung, kidney, skeletal muscle, adipose and brain). Using single-nucleotide polymorphism (SNP)-based method, was identified to have monoallelic expression in adult tissues, suggesting that it is imprinted in cows. These results provide a foundation for further investigation about whether plays a role in regulating imprinting of the domain.
长链非编码RNA（lncRNAs）是生物过程的重要调节因子，在……中调节基因组印记和/或诱导具有亲本来源特异性模式的单等位基因表达。……结构域是哺乳动物中最大的印记簇之一，该区域母源表达的非编码RNA与胚胎干细胞的多能性有关。此前，我们对两个母源表达的非编码RNA……和……的cDNA进行了测序，并在21号染色体牛……结构域的两个基因之间定位了一个lncRNA（……）。在本研究中，我们鉴定了一种新型的长链基因间非编码RNA（lincRNA）……，它也位于……和……之间。我们鉴定出LINC24065的四个变体（……、……、……和……），它们是由18个外显子的可变剪接产生的。……和……在成年牛组织中表现出组织特异性表达模式，并且在所有八个分析组织（心脏、肝脏、脾脏、肺、肾脏、骨骼肌、脂肪和大脑）中都检测到了……和……。使用基于单核苷酸多态性（SNP）的方法，确定……在成年组织中具有单等位基因表达，表明它在奶牛中是印记的。这些结果为进一步研究……是否在调节……结构域的印记中发挥作用提供了基础。
