Synergetic Innovation Center for Quantum Effects and Application, Key Laboratory of Low-dimensional Quantum Structures and Quantum Control of Ministry of Education, School of Physics and Electronics, Hunan Normal University, Changsha 410081, PR China.
Synergetic Innovation Center for Quantum Effects and Application, Key Laboratory of Low-dimensional Quantum Structures and Quantum Control of Ministry of Education, School of Physics and Electronics, Hunan Normal University, Changsha 410081, PR China.
Mater Sci Eng C Mater Biol Appl. 2019 Jul;100:260-268. doi: 10.1016/j.msec.2019.02.106. Epub 2019 Feb 28.
Optical bioimaging in second near-infrared window (NIR-II, 1000-1700 nm) has been emerged as an indispensable tool for highly sensitive disease detection. In this work, intense NIR-II emissive polyacrylic acid (PAA) modified NaLuF: Gd/Nd nanorods (PAA-NRs) with pure hexagonal phase and uniform size were explored for high sensitivity in vivo NIR-II bioimaging and optical imaging-guided small tumor detection. The NIR-II emission of the NaLuF: Gd host can be readily adjusted by doping Nd, making it promising emission centered at 1056 nm and 1328 nm with high photo-stability. The time-dependent in vivo tracking results validate that the PAA-NRs are mainly accumulated in the reticuloendothelial system (RES) and excreted through the hepatic pathway. In addition, NIR-II optical imaging-guided small tumor (down to 5 mm) diagnosis was successfully achieved. Remarkably, in vivo small blood vessel with high spatial resolution (~105 μm) was detected clearly. And the histological tests reveal that our designed hydrophilic NRs present negligible toxicity effects and good biocompatibility in living animals. Besides the NIR-II emission, the PAA-NRs also present X-ray absorption features for X-ray bioimaging. These findings demonstrate that the explored lanthanide-based NRs with controllable size, efficient NIR-II emission and decent biocompatibility are promising NIR-II contrast agents for future biomedical applications, such as, early diagnosis of small tumor, vascular related disease imaging and angiogenesis diagnosis.
在近红外二区（NIR-II，1000-1700nm）的光学生物成像已经成为高灵敏度疾病检测不可或缺的工具。在这项工作中，我们探索了强近红外二区发射的聚丙烯酸（PAA）修饰的 NaLuF:Gd/Nd 纳米棒（PAA-NRs），其具有纯六方相和均匀的尺寸，可实现高灵敏度的体内近红外二区生物成像和光学成像引导的小肿瘤检测。NaLuF:Gd 基质的近红外二区发射可以通过掺杂 Nd 来轻松调节，使其有望发射出中心位于 1056nm 和 1328nm 的高稳定性光。时间依赖性体内追踪结果验证了 PAA-NRs 主要在网状内皮系统（RES）中积累，并通过肝途径排泄。此外，成功实现了近红外二区光学成像引导的小肿瘤（小至 5mm）诊断。值得注意的是，体内小血管的高空间分辨率（~105μm）被清晰地检测到。组织学测试表明，我们设计的亲水性 NRs 在活体动物中表现出极小的毒性作用和良好的生物相容性。除了近红外二区发射外，PAA-NRs 还具有 X 射线生物成像的 X 射线吸收特性。这些发现表明，所探索的具有可控尺寸、高效近红外二区发射和良好生物相容性的基于镧系元素的 NRs 是有前途的近红外二区对比剂，可用于未来的生物医学应用，例如小肿瘤的早期诊断、血管相关疾病成像和血管生成诊断。
