Centre for Materials Innovation and Future Fashion, School of Fashion and Textiles, College of Design and Social Context, RMIT University, Brunswick 3056, Australia.
Functional, Innovative and Smart Textiles, PSG Institute of Advanced Studies, Coimbatore 641004, India.
Mater Sci Eng C Mater Biol Appl. 2019 Jul;100:378-387. doi: 10.1016/j.msec.2019.02.110. Epub 2019 Mar 2.
We describe preparation, characterization and cytocompatibility of nanodiamond (ND) dispersed in poly (ε-caprolactone) (PCL) based nanofibrous scaffold. The results show that this unique scaffold potentially provides essential properties for wound healing by enhancing proliferation of epithelial cells, in addition to restricting the microbial activities. Electrospinning technique was used to fabricate and develop PCL-NDs nanocomposite scaffold. The developed nanocomposites were characterized for morphology, thermal, surface and biological properties. The incorporation of ND into the PCL matrix resulted in better moisture management and higher thermal stability. Transmission electron microscopy images and attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy showed existence of ND particles on the surface of the nanofibers. The aggregation of ND particles increased with the increase in their concentration in nanofiber. The developed scaffolds showed no cytotoxicity and, due to improved hydrophilicity, better cellular activities with Chinese hamster ovarian (CHO) cells, 43%, 38% and 22% more cell proliferation for PCL-5% ND for 1, 3- and 7-days incubations in compare with PCL. Furthermore, Staphylococcus aureus (S. aureus) showed significantly less affinity to the scaffold surface with the increase in ND concentration, ~56% less for PCL-5% ND in compare with PCL, indicating that such ND dispersed nanofibrous scaffold maybe asuitable choice for complex wound management.
我们描述了纳米金刚石 (ND) 在聚己内酯 (PCL) 基纳米纤维支架中分散的制备、表征和细胞相容性。结果表明，这种独特的支架通过促进上皮细胞的增殖，除了限制微生物的活动外，还有可能为伤口愈合提供必要的特性。静电纺丝技术用于制备和开发 PCL-NDs 纳米复合材料支架。对开发的纳米复合材料进行了形态、热、表面和生物性能的表征。ND 掺入 PCL 基体中导致更好的水分管理和更高的热稳定性。透射电子显微镜图像和衰减全反射傅里叶变换红外（ATR-FTIR）光谱显示 ND 颗粒存在于纳米纤维的表面上。ND 颗粒的聚集随着其在纳米纤维中的浓度的增加而增加。与 PCL 相比，所开发的支架无细胞毒性，由于亲水性提高，与中国仓鼠卵巢（CHO）细胞的细胞活性更好，在 1、3 和 7 天孵育时，PCL-5%ND 的细胞增殖率分别提高了 43%、38%和 22%。此外，随着 ND 浓度的增加，金黄色葡萄球菌（S. aureus）对支架表面的亲和力显著降低，PCL-5%ND 比 PCL 降低约 56%，表明这种 ND 分散的纳米纤维支架可能是复杂伤口管理的合适选择。
