Alharbi Amjad, Toulah Fawzia H, Wakid Majed H, Azhar Esam, Farraj Suha, Mirza Ahmed A
Faculty of Science for Girls, Department of Biology, King Abdulaziz University, Jeddah, SAU.
College of Science, Department of Biology, University of Jeddah, Jeddah, SAU.
Cureus. 2020 Sep 7;12(9):e10287. doi: 10.7759/cureus.10287.
Background is a pathogenic intestinal flagellate transmitted by the ingestion of contaminated water or food with the cyst stage of the parasite. Giardiasis can cause severe acute diarrhea and malabsorption or may persist as a chronic infection. Effective treatment and control measures depend on proper laboratory diagnosis using diagnostic methods with high sensitivity and specificity. Objective To compare the sensitivity and specificity of direct smear, Ritchie sedimentation technique, two brands of rapid chromatographic immunoassay test, and real-time polymerase chain reaction (PCR) for the detection of in clinical human fecal samples. Materials and methods Unpreserved 100 stool specimens were collected in clean plastic containers and labeled with the patient's information and examined through light microscopy, immunochromatographic test (ICTs), and real-time PCR. Results Out of 100 fresh stool samples obtained from workers analyzed, real-time PCR targeting the SSU rRNA gene was able to detect deoxyribonucleic acid (DNA) in (42) samples followed by ImmunoCard STAT! (31) samples (Meridian Bioscience, Germany), direct smear (23) samples, CerTest (19) samples (Biotec, Zaragoza, Spain), and Ritchie technique (17) samples. Real-time PCR was the most sensitive for the diagnosis of in comparison to the other techniques. Conclusions All the techniques investigated were sensitive for the detection of in stool samples. Further studies are recommended using multiplex real-time PCR assay in order to increase the possibility of the presence or absence of the infection.
背景 是一种致病性肠道鞭毛虫，通过摄入被寄生虫包囊阶段污染的水或食物传播。贾第虫病可导致严重的急性腹泻和吸收不良，或可能持续为慢性感染。有效的治疗和控制措施取决于使用具有高灵敏度和特异性的诊断方法进行正确的实验室诊断。目的 比较直接涂片法、里奇沉淀技术、两种品牌的快速色谱免疫分析试验和实时聚合酶链反应（PCR）检测临床人类粪便样本中 的灵敏度和特异性。材料和方法 用干净的塑料容器收集100份未保存的粪便标本，标上患者信息，并通过光学显微镜、免疫色谱试验（ICTs）和实时PCR进行检测。结果 在从接受分析的工人中获得的100份新鲜粪便样本中，针对小亚基核糖体RNA（SSU rRNA）基因的实时PCR能够在42份样本中检测到 脱氧核糖核酸（DNA），其次是免疫卡STAT！（31份）样本（德国子午线生物科学公司）、直接涂片（23份）样本、CerTest（19份）样本（西班牙萨拉戈萨的Biotec公司）和里奇技术（17份）样本。与其他技术相比，实时PCR对 的诊断最敏感。结论 所有研究的技术对粪便样本中 的检测都很敏感。建议进一步使用多重实时PCR检测法，以增加感染存在与否的可能性。
