Zarei Omid, Arabestan Mohammad Reza, Majlesi Amir, Mohammadi Younes, Alikhani Mohammad Yousef
Department of Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
Department of Gastroenterology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
Gastroenterol Hepatol Bed Bench. 2019 Winter;12(1):52-59.
This study determined the genes encoding the binding and receiving factors of iron and microbial biofilm in strains isolated from mucosal samples of patients with colorectal cancer and inflammation of the colorectal compared to healthy people.
Colorectal cancer is one of the most important malignancies in recent years. is the most important infectious agents associated with colorectal cancer that has numerous virulence factors such as iron uptake and adhesion factors included in the process of inflammation and colorectal cancer.
Of the three healthy, inflammatory bowel diseases and colorectal cancer groups, 40 strains isolated after confirmation by biochemical and molecular methods. After determining the isolates phylogroups, the frequency of genes was measured by PCR method. The biofilm formation of isolates was performed using Crystal Violet method.
In the determination of the bacteria phylogroups, the colorectal cancer isolates had a maximum incidence of phylogroups B2 and A. In the analysis of , , , , and the highest frequency was observed in these two phylogroups. The presence of gene in bacterial isolates from three groups showed a significant difference ( value: 0.004). There was also no significant difference in biofilm productions in strains isolated from the three groups.
Unlike previous studies focusing solely on toxins, we found that iron absorption and adhesion factors could be effective in developing inflammatory bowel diseases and colorectal cancer. It was also determined that biofilm formation is a specific characteristic of isolated from the healthy colon.
本研究确定了从结直肠癌患者和结肠炎症患者的黏膜样本中分离出的菌株中编码铁和微生物生物膜结合及接收因子的基因，并与健康人进行比较。
结直肠癌是近年来最重要的恶性肿瘤之一。是与结直肠癌相关的最重要的感染因子，其具有多种毒力因子，如参与炎症和结直肠癌过程的铁摄取和粘附因子。
在健康、炎症性肠病和结直肠癌三组中，经生化和分子方法确认后分离出40株菌株。确定分离株的系统发育群后，采用PCR方法检测基因频率。采用结晶紫法检测分离株的生物膜形成情况。
在细菌系统发育群的测定中，结直肠癌分离株中系统发育群B2和A的发生率最高。在对、、、、和的分析中，这两个系统发育群的频率最高。三组细菌分离株中基因的存在情况存在显著差异（值：0.004）。从三组中分离出的菌株的生物膜产生情况也没有显著差异。
与以往仅关注毒素的研究不同，我们发现铁吸收和粘附因子可能在炎症性肠病和结直肠癌的发生发展中起作用。还确定生物膜形成是从健康结肠分离出的菌株的一个特定特征。
