Khan Shahanavaj, Zaidi Sabika, Alouffi Abdulaziz Saleh, Hassan Iftekhar, Imran Ahmad, Khan Rais Ahmad
Bioinformatics and Biotechnology Unit, Department of Biosciences, SRGC, Muzaffarnagar 251001, UP, India.
Department of Pharmaceutics, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia.
ACS Omega. 2020 Mar 30;5(13):7254-7261. doi: 10.1021/acsomega.9b04042. eCollection 2020 Apr 7.
Enterohemorrhagic infection is associated with gastrointestinal disorders, including diarrhea and colorectal cancer. Although evidences have established the involvement of in the growth of colon cancer, the molecular mechanisms of carcinogenesis of cancer growth and development are not well understood. We analyzed protein targeting in host cell organelles and the implication in colon cancer using in silico approaches. Our results indicated that many proteins targeted the endoplasmic reticulum (ER), ER membranes, Golgi apparatus, Golgi apparatus membranes, peroxisomes, nucleus, nuclear membrane, mitochondria, and mitochondrial membrane of host cells. These targeted proteins in ER, Golgi apparatus, peroxisomes, nucleus, and mitochondria may alter the normal functioning of various pathways including DNA repair, apoptosis, replication, transcription, and protein folding in -infected host cells. The results of the current in silico study provide insights into pathogenesis and may aid in designing new preventive and therapeutic strategies.
肠出血性感染与胃肠道疾病有关，包括腹泻和结直肠癌。尽管已有证据表明其与结肠癌的生长有关，但癌症生长和发展的致癌分子机制尚未完全明确。我们使用计算机模拟方法分析了宿主细胞器中的蛋白质靶向作用及其在结肠癌中的意义。我们的结果表明，许多蛋白质靶向宿主细胞的内质网（ER）、内质网膜、高尔基体、高尔基体膜、过氧化物酶体、细胞核、核膜、线粒体和线粒体膜。这些在内质网、高尔基体、过氧化物酶体、细胞核和线粒体中的靶向蛋白质可能会改变感染宿主细胞中包括DNA修复、凋亡、复制、转录和蛋白质折叠在内的各种途径的正常功能。当前计算机模拟研究的结果为该疾病的发病机制提供了见解，并可能有助于设计新的预防和治疗策略。
