Messaili Chahinez, Messai Yamina, Bakour Rabah
Laboratory of Cellular and Molecular Biology, Faculty of Biological Sciences,University of Sciences and Technology Houari Boumediene, PB 32 El-Alia, Bab Ezzouar, 16111 Algiers, Algeria.
Vet Ital. 2019 Mar 31;55(1):35-46. doi: 10.12834/VetIt.799.3865.2.
The objective of the study was to determine the virulence and antimicrobial resistance traits of 100 fecal E. coli strains isolated from clinically healthy chickens in Algeria. Most of isolates belonged to phylogroups A (45%) and B1 (37%) and showed a great diversity in DNA profiles. The genes fimH, tsh, entB, iutA, irp2, fyuA, iroN, sitA, etsA, etsB, eitA, iss, traT, ompT, hlyF, vat, ibeA, cvaA, cvaB5', cvaB3', cvaC, cma and cbi were detected. Combinations of virulence genes defined 67 virulence profiles. High resistance rates (62‑97%) were noted for amoxicillin, amoxicillin‑clavulanic acid, cefazolin, fluoroquinolones, tetracycline, trimethoprim, sulfonamides and sulfamethoxazole/ trimethoprim, and 93% of strains were multidrug‑resistant. Combinations of resistance phenotypes defined 59 resistance patterns. The genes blaTEM, blaSHV, blaCTX‑M‑1, tetA, tetB, qnrB, qnrS1, sul1, sul2, sul3, dfrA1, dfrA7, dfrA12 and dfrA14 were identified and class 1 integrons were detected in 49% of isolates. A rate of 37% of strains was resistant to mercury, with the presence of merA gene. The study reports the presence in the avian strains isolated from fecal swabs of virulence genes of plasmid origin characteristic of ExPEC strains associated with high resistance to first‑line antibiotics and class 1 integrons, this augurs a risk for human and animal health.
该研究的目的是确定从阿尔及利亚临床健康鸡中分离出的100株粪便大肠杆菌菌株的毒力和抗菌耐药性特征。大多数分离株属于A群（45%）和B1群（37%），并且在DNA图谱上表现出很大的多样性。检测到了fimH、tsh、entB、iutA、irp2、fyuA、iroN、sitA、etsA、etsB、eitA、iss、traT、ompT、hlyF、vat、ibeA、cvaA、cvaB5'、cvaB3'、cvaC、cma和cbi等基因。毒力基因的组合定义了67种毒力谱。阿莫西林、阿莫西林-克拉维酸、头孢唑林、氟喹诺酮类、四环素、甲氧苄啶、磺胺类和磺胺甲恶唑/甲氧苄啶的耐药率较高（62%-97%），93%的菌株对多种药物耐药。耐药表型的组合定义了59种耐药模式。鉴定出了blaTEM、blaSHV、blaCTX-M-1、tetA、tetB、qnrB、qnrS1、sul1、sul2、sul3、dfrA1、dfrA7、dfrA12和dfrA14等基因，49%的分离株中检测到1类整合子。37%的菌株对汞耐药，存在merA基因。该研究报告称，从粪便拭子中分离出的禽类菌株中存在源自质粒的毒力基因，这些基因是与对一线抗生素和1类整合子高度耐药相关的肠外致病性大肠杆菌菌株的特征，这预示着对人类和动物健康存在风险。
