Glukhova M A, Kabakov A E, Belkin A M, Frid M G, Ornatsky O I, Zhidkova N I, Koteliansky V E
FEBS Lett. 1986 Oct 20;207(1):139-41. doi: 10.1016/0014-5793(86)80027-8.
Meta-vinculin distribution in adult human tissue was studied by immunoblotting technique. Meta-vinculin was found in smooth (aorta wall and myometrium) and cardiac muscle, rather than in skeletal muscle, liver, kidney and cultured cells - macrophages, foreskin fibroblasts, peripheral blood lymphocytes and vascular endothelial cells. In the primary culture of smooth muscle cells from human aorta the meta-vinculin/vinculin ratio was reduced, and on the onset of cell division meta-vinculin could hardly be detected. Subcultured smooth muscle cells from human aorta did not contain meta-vinculin. The data show that the presence of meta-vinculin is characteristic of 'contractile' smooth muscle cells rather than of proliferating in vitro.
采用免疫印迹技术研究了成人组织中变构纽蛋白的分布。在平滑肌（主动脉壁和子宫肌层）和心肌中发现了变构纽蛋白，而在骨骼肌、肝脏、肾脏以及培养细胞——巨噬细胞、包皮成纤维细胞、外周血淋巴细胞和血管内皮细胞中未发现。在人主动脉平滑肌细胞的原代培养中，变构纽蛋白/纽蛋白的比例降低，并且在细胞分裂开始时几乎检测不到变构纽蛋白。来自人主动脉的传代培养平滑肌细胞不含变构纽蛋白。数据表明，变构纽蛋白的存在是“收缩性”平滑肌细胞的特征，而非体外增殖细胞的特征。
