Laudan R, Stolen J S, Cali A
Dev Comp Immunol. 1986 Summer;10(3):331-40. doi: 10.1016/0145-305x(86)90023-6.
The microsporidan parasite G. stephani, has been found in the intestine of the American winter flounder Pseudopleuronectes americanus. A quantitative study of total serum immunoglobulin (Ig) levels has shown that an injection of this parasitic spore causes a decrease in serum IgM levels. The level of suppression seems to be related to the number of spores injected into the host, as well as the frequency of injections administered. As the dosage of spores injected into the winter flounder increased from 3 X 10(3)/ml to 3 X 10(8)/ml, the level of serum IgM's decreased proportionally. When a single IM injection of spores (3 X 10(6)/ml) was administered, the initial decrease in serum IgM was followed by a gradual recovery of total Ig levels by day 60. Repeated injections of G. stephani on days 21 and 42 respectively, causes an even greater decrease in total IgM levels. Indomethacin (a drug that inhibits prostaglandin activity), has been shown to negate the decrease in IgM levels caused by the G. stephani spores. When injected twice weekly, the indomethacin inhibited the decrease in serum IgM caused by the spore homogenate, with IgM levels comparable to those observed in control WF. This supports previous observations, that prostaglandins are implicated in the decrease in IgM levels induced by injection of the parasite. The effects of G. stephani on serum Ig levels does not seem to be species-specific. When the spores were injected into summer flounder (Paralichthys dentatus) together with an erythrocyte antigen, horse red blood cells (HRBC), a decrease in total IgM levels occurred, as well as a decrease in hemagglutination titers to HRBC similar to the decrease described in winter flounder.
已在美国冬鲽（Pseudopleuronectes americanus）的肠道中发现了微孢子虫寄生虫斯蒂芬格氏微孢子虫（G. stephani）。一项关于血清总免疫球蛋白（Ig）水平的定量研究表明，注射这种寄生孢子会导致血清IgM水平下降。抑制程度似乎与注入宿主体内的孢子数量以及注射频率有关。随着注入冬鲽体内的孢子剂量从3×10³/ml增加到3×10⁸/ml，血清IgM水平成比例下降。当单次肌肉注射孢子（3×10⁶/ml）时，血清IgM最初下降，随后在第60天时总Ig水平逐渐恢复。分别在第21天和第42天重复注射斯蒂芬格氏微孢子虫，会导致总IgM水平出现更大幅度的下降。消炎痛（一种抑制前列腺素活性的药物）已被证明可消除斯蒂芬格氏微孢子虫孢子引起的IgM水平下降。当每周注射两次时，消炎痛抑制了孢子匀浆引起的血清IgM下降，IgM水平与对照冬鲽中观察到的水平相当。这支持了先前的观察结果，即前列腺素与注射寄生虫诱导的IgM水平下降有关。斯蒂芬格氏微孢子虫对血清Ig水平的影响似乎不是物种特异性的。当将孢子与红细胞抗原马红细胞（HRBC）一起注射到夏季比目鱼（Paralichthys dentatus）体内时，总IgM水平下降，同时对HRBC的血凝滴度也下降，这与冬鲽中描述的下降情况类似。
