洋葱皮槲皮素负载壳聚糖-纤维素水凝胶与绿色氧化锌纳米粒子的协同配方，实现可控释放、生物相容性、抗菌和抗癌活性。
Synergic formulation of onion peel quercetin loaded chitosan-cellulose hydrogel with green zinc oxide nanoparticles towards controlled release, biocompatibility, antimicrobial and anticancer activity.
机构信息
Department of Chemical Engineering, National Institute of Technology, Tiruchirapalli 620015, Tamilnadu, India.
Department of Chemical Engineering, National Institute of Technology, Tiruchirapalli 620015, Tamilnadu, India.
出版信息
Int J Biol Macromol. 2019 Jul 1;132:784-794. doi: 10.1016/j.ijbiomac.2019.04.008. Epub 2019 Apr 2.
The therapeutic prospective of a novel carrier based delivery of phyto-derived quercetin (QE) extracted from onion peel waste was studied in the present work. Dialdehyde cellulose (DAC) developed from sugarcane bagasse (SCB) cellulose effectively crosslinked chitosan hydrogel. The hydrogel matrices were embedded with green zinc oxide nanoparticles (ZnO NPs), phyto-synthesized using musk melon seeds. The hybrid carrier formulation was characterised using analytical techniques such as XRD, FTIR and SEM analysis. The onion peel drug (OPD) analysed for its bioactive components using HPLC was reported as a potential source of QE. Taguchi optimization for the QE drug loading in the nanohybrid hydrogel indicated a remarkable improvement by 39% in comparison to drug loading in hydrogel without ZnO NPs. The maximum QE release was obtained at an optimal drug loading condition with pH 5.0, which favoured the anticancer applications. The drug release revealed a Fickian diffusion mechanism by adopting various kinetic models. The commercial QE and QE in OPD (QE/OPD) loaded nanohybrid hydrogels were found to inhibit the growth of Staphylococcus aureus and Trichophyton rubrum strains. The biocompatibility and anticancer properties of the QE/OPD loaded nanohybrid hydrogels were established against normal L929 murine fibroblast cells and A431 human skin carcinoma cell lines respectively.
本工作研究了一种新型载体递送植物源槲皮素（QE）的治疗前景，该 QE 是从洋葱皮废物中提取的。用甘蔗渣（SCB）纤维素制备的二醛纤维素（DAC）可有效交联壳聚糖水凝胶。将绿色氧化锌纳米粒子（ZnO NPs）嵌入水凝胶基质中，该 ZnO NPs 是使用甜瓜种子植物合成的。采用 XRD、FTIR 和 SEM 分析等分析技术对混合载体配方进行了表征。使用 HPLC 对洋葱皮药物（OPD）进行了生物活性成分分析，报告称其为 QE 的潜在来源。通过 Taguchi 优化，纳米复合水凝胶中的 QE 药物载量显著提高了 39%，与不含 ZnO NPs 的水凝胶中的药物载量相比。在 pH 5.0 的最佳药物载量条件下，可获得最大的 QE 释放量，有利于抗癌应用。通过采用各种动力学模型，药物释放显示出菲克扩散机制。发现商业 QE 和 QE 在 OPD（QE/OPD）负载纳米复合水凝胶中能够抑制金黄色葡萄球菌和红色毛癣菌的生长。QE/OPD 负载纳米复合水凝胶的生物相容性和抗癌特性分别通过对正常 L929 鼠成纤维细胞和 A431 人皮肤癌细胞系进行了评估。
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