Department of Biology, College of Sciences, Shiraz University, Shiraz, Iran.
Department of Biology, College of Sciences, Shiraz University, Shiraz, Iran.
Gene. 2019 Jun 30;703:13-16. doi: 10.1016/j.gene.2019.04.003. Epub 2019 Apr 3.
Today several millions polymorphic sites in human genome are well described. Many investigators are studying the association between these polymorphisms and susceptibility to multifactorial traits. These polymorphisms are also used for studying the population's genetic structures. Here, we introduce a new simple one step method for estimating the allelic frequency of polymorphic sites in pooled samples. The method is based on measurement of the intensity of polymorphic bands on agarose gel electrophoresis. This method is very simple, rapid, inexpensive, and is more sensitive compared to the chip-based matrix-assisted laser desorption/ionization time-of-flight mass spectrometry method.
如今，人类基因组中已有数百万个多态性位点得到了很好的描述。许多研究人员正在研究这些多态性与多因素性状易感性之间的关系。这些多态性也被用于研究人群的遗传结构。在这里，我们介绍了一种新的简单一步法，用于估计混合样本中多态性位点的等位基因频率。该方法基于琼脂糖凝胶电泳中多态性带强度的测量。与基于芯片的基质辅助激光解吸/电离飞行时间质谱法相比，该方法非常简单、快速、廉价，并且更灵敏。
