枯草芽孢杆菌噬菌体PZA右早期区域的核苷酸序列完善了PZA基因组19366 bp的序列。与噬菌体phi 29的同源序列比较。

Nucleotide sequence of the right early region of Bacillus subtilis phage PZA completes the 19366-bp sequence of PZA genome. Comparison with the homologous sequence of phage phi 29.

作者信息

Paces V, Vlcek C, Urbánek P, Hostomský Z

出版信息

Gene. 1986;44(1):115-20. doi: 10.1016/0378-1119(86)90049-1.

Abstract

We have sequenced the rightmost 2079 bp of the Bacillus subtilis phage PZA genome. This region encompasses the right early region. We compared it with the homologous region of phage phi 29. Six open reading frames (ORFs) were found in this region of PZA and one of them was assigned to gene 17. Analysis of putative ribosome-binding sites and comparison with phi 29 ORFs indicate that at least some of the remaining ORFs could encode proteins. Corresponding genes were not identified so far by genetic methods. Promoter candidates in the right early region of PZA were found and compared to phi 29 promoters. The sequenced region together with previously determined sequences [Paces et al., Gene 38 (1985) 45-56 and 44 (1986) 107-114] completes the entire 19,366-bp sequence of phage PZA genome.

摘要

我们已对枯草芽孢杆菌噬菌体PZA基因组最右侧的2079个碱基对进行了测序。该区域包含右早期区域。我们将其与噬菌体phi 29的同源区域进行了比较。在PZA的该区域发现了六个开放阅读框（ORF），其中一个被指定为基因17。对推定核糖体结合位点的分析以及与phi 29 ORF的比较表明，其余的ORF中至少有一些可能编码蛋白质。到目前为止，通过遗传方法尚未鉴定出相应的基因。在PZA的右早期区域发现了启动子候选序列，并与phi 29启动子进行了比较。测序区域与先前确定的序列[Paces等人，《基因》38（1985）45 - 56和44（1986）107 - 114]共同构成了噬菌体PZA基因组完整的19366个碱基对序列。