Steiner M, Lubitz W, Bläsi U
Institute of Microbiology and Genetics, University of Vienna, Austria.
J Bacteriol. 1993 Feb;175(4):1038-42. doi: 10.1128/jb.175.4.1038-1042.1993.
In most bacteriophages of gram-negative bacteria, the phage endolysin is released to its murein substrate through a lesion in the inner membrane. The lesion is brought about by a second phage-encoded lysis function. For the first time, we present evidence that the same strategy is elaborated by a phage of a gram-positive bacterium. Thus, there appears to be an evolutionarily conserved lysis pathway for most phages whether their host bacterium is gram negative or gram positive. Phage phi 29 gene 14, the product of which is required for efficient lysis of Bacillus subtilis, was cloned in Escherichia coli. Production of protein 14 in E. coli resulted in cell death, whereas production of protein 14 concomitantly with the phi 29 lysozyme or unrelated murein-degrading enzymes led to lysis, suggesting that membrane-bound protein 14 induces a nonspecific lesion in the cytoplasmic membrane.
在大多数革兰氏阴性菌噬菌体中，噬菌体溶菌酶通过内膜损伤释放到其胞壁质底物上。这种损伤是由第二种噬菌体编码的裂解功能引起的。我们首次提供证据表明，革兰氏阳性菌的一种噬菌体也采用了相同的策略。因此，无论宿主细菌是革兰氏阴性还是革兰氏阳性，大多数噬菌体似乎都有一条进化上保守的裂解途径。噬菌体φ29基因14的产物是枯草芽孢杆菌有效裂解所必需的，该基因已在大肠杆菌中克隆。在大肠杆菌中产生蛋白质14会导致细胞死亡，而蛋白质14与φ29溶菌酶或无关的胞壁质降解酶同时产生则会导致裂解，这表明膜结合蛋白14会在细胞质膜上诱导非特异性损伤。
