Cully D F, Garro A J
Gene. 1985;38(1-3):153-64. doi: 10.1016/0378-1119(85)90214-8.
A gene involved in the regulation of lysogeny in the temperate Bacillus subtilis phage phi 105 has been identified and isolated. A plasmid, pDC4, was constructed that contains a 740-bp HindIII-PvuII fragment that is derived from the phi 105 immunity region and is capable of rendering B. subtilis immune to infection by phi 105. Three different hybrid plasmids that contain the 740-bp fragment, pAG101 [Cully and Garro, J. Virol. 34 (1980) 789-791], pDC1 and pDC2, were found to synthesize a common 18-kDal polypeptide in B. subtilis minicells and Escherichia coli maxicells. The nucleotide (nt) sequence of this region revealed three open reading frames (ORFs) that predict proteins with Mrs of 16521, 7332, and 5516. In vivo synthesized phi 105 prophage RNA was mapped by primer extension and shown to be transcribed from the DNA strand coding for the Mr 16521 protein. The 5' end of the phi 105 lysogen RNA was mapped to a region that contains conserved sequences for RNA polymerase recognition.
已鉴定并分离出一种参与温和型枯草芽孢杆菌噬菌体phi 105溶原性调控的基因。构建了一种质粒pDC4，它含有一个740 bp的HindIII - PvuII片段，该片段来源于phi 105免疫区域，能够使枯草芽孢杆菌对phi 105感染产生免疫。发现三种含有740 bp片段的不同杂交质粒pAG101 [Cully和Garro，《病毒学杂志》34 (1980) 789 - 791]、pDC1和pDC2在枯草芽孢杆菌微小细胞和大肠杆菌大细胞中合成一种共同的18 kDa多肽。该区域的核苷酸(nt)序列揭示了三个开放阅读框(ORF)，它们预测的蛋白质分子量分别为16521、7332和5516。通过引物延伸对体内合成的phi 105原噬菌体RNA进行定位，结果表明它是从编码分子量为16521的蛋白质的DNA链转录而来。phi 105溶原RNA的5'端定位到一个含有RNA聚合酶识别保守序列的区域。
