Programa de Pós-graduação em Biociências e Fisiopatologia, Universidade Estadual de Maringá, Av. Colombo, n° 5790, CEP: 87020-900 Maringá, Paraná, Brazil.
Universidade Federal do Recôncavo da Bahia, Av. Carlos Amaral, Cajueiro, CEP 44574-490, Santo Antônio de Jesus, BA; and Universidade Federal da Bahia, Instituto de Ciências da Saúde, Av. Reitor Miguel Calmon, Vale do Canela, Salvador, BA, Brazil.
Biomed Pharmacother. 2019 Jun;114:108797. doi: 10.1016/j.biopha.2019.108797. Epub 2019 Apr 2.
To analyze the remodeling dynamics of total collagen, type I and III, the expression of ICAM-1 and 5-HT in the jejunum of rats.
Twenty-eight Wistar rats were randomly assigned to two experimental groups: the control group (CG, n = 7) and the infected group (receiving 5,000 sporulated T. gondii oocysts - ME49 strain, genotype II, n = 21). Seven infected rats each at 6 (G6), 12 (G12), and 24 (G24) hours post infection were sacrificed and segments of jejunum were collected for standard histological, histochemical, and immunohistochemistry processing techniques.
The infection promoted ICAM-1 and 5-HT expression, type III collagen, and total mast cell increases. However, it also caused a reduction in the area occupied by type I collagen fibers, and in submucosa thickness, and caused ganglion and peri-ganglion alterations.
The structural damage caused by toxoplasmic infection is intense during the first 24 h post inoculation. At peak dissemination, from 12 to 24 h, there is an increase in ICAM-1 and 5-HT expression, with intense migration of mast cells to the site of infection. There was also a reduction in submucosa thickness, and an effective loss of extracellular matrix (ECM) organization, which included changes in the dynamics of type I and III total collagen deposition.
分析大鼠空肠总胶原、I 型和 III 型的重塑动态，以及细胞间黏附分子-1（ICAM-1）和 5-羟色胺（5-HT）的表达。
将 28 只 Wistar 大鼠随机分为两组：对照组（CG，n=7）和感染组（感染 5000 个孢子化 T. gondii 卵囊-ME49 株，基因型 II，n=21）。每组感染后 6（G6）、12（G12）和 24（G24）小时各处死 7 只大鼠，取空肠段进行标准组织学、组织化学和免疫组织化学处理。
感染促进了 ICAM-1 和 5-HT 的表达、III 型胶原和总肥大细胞的增加。然而，它也导致 I 型胶原纤维面积减少、黏膜下层厚度减少，并导致神经节和神经节周围改变。
在接种后 24 小时内，弓形虫感染导致的结构损伤非常严重。在传播高峰期，即 12 至 24 小时，ICAM-1 和 5-HT 的表达增加，肥大细胞强烈迁移到感染部位。黏膜下层厚度减少，细胞外基质（ECM）组织有效丧失，包括 I 型和 III 型总胶原沉积的动态变化。
