Key Laboratory of Micro-systems and Micro-structures Manufacturing, Ministry of Education and School of Mechatronics Engineering, Harbin Institute of Technology, Harbin, Heilongjiang, 150010, China.
Key Laboratory of Micro-systems and Micro-structures Manufacturing, Ministry of Education and School of Mechatronics Engineering, Harbin Institute of Technology, Harbin, Heilongjiang, 150010, China.
Biosens Bioelectron. 2019 Jun 1;134:16-23. doi: 10.1016/j.bios.2019.03.053. Epub 2019 Mar 29.
Saliva has been reported to contain various cytokine biomarkers which are associated with some severe diseases such as cancers. Non-invasive saliva diagnosis using wearable or portable devices may pave a new avenue for monitoring conditions of the high risk population. Here, a graphene-based fully integrated portable nanosensing system, the entire size of which is smaller than a smart-phone and can be handheld, is presented for on-line detection of cytokine biomarkers in saliva. This miniaturized system employs an aptameric graphene-based field effect transistor (GFET) using a buried-gate geometry with HfO as the dielectric layer and on-line signal processing circuits to realize the transduction and processing of signals which reflect cytokine concentrations. The signal can be wirelessly transmitted to a smart-phone or cloud sever through the Wi-Fi connection for visualizing the trend of the cytokine concentration change. Interleukin-6 (IL-6) is used as a representative to examine the sensing capability of the system. Experimental results demonstrate that the nanosensing system responds to the change of IL-6 concentration within 400s in saliva with a detection limit down to 12 pM. Therefore, this portable system offers the practicality to be potentially used for non-invasive saliva diagnosis of diseases at early stage.
唾液中含有各种细胞因子生物标志物，这些标志物与一些严重疾病（如癌症）有关。使用可穿戴或便携式设备进行非侵入性唾液诊断，可能为监测高危人群的健康状况开辟新途径。在这里，我们提出了一种基于石墨烯的完全集成的便携式纳米传感系统，其整体尺寸小于智能手机，可手持使用，可用于在线检测唾液中的细胞因子生物标志物。该微型系统采用基于适体的石墨烯场效应晶体管（GFET），采用埋栅几何结构，以 HfO 作为介电层，并采用在线信号处理电路，实现反映细胞因子浓度的信号转换和处理。信号可以通过 Wi-Fi 连接无线传输到智能手机或云服务器，以可视化细胞因子浓度变化的趋势。白细胞介素-6（IL-6）被用作代表来检查系统的传感能力。实验结果表明，该纳米传感系统可在 400s 内响应唾液中 IL-6 浓度的变化，检测限低至 12 pM。因此，该便携式系统具有用于早期非侵入性唾液诊断疾病的实用性。
