Wong Daniel E, Banyard Derek A, Santos Pauline J F, Sayadi Lohrasb R, Evans Gregory R D, Widgerow Alan D
Warren Alpert Medical School of Brown University, Providence, RI, United States.
Department of Plastic Surgery, University of California, Irvine, Orange, CA, United States; Center for Tissue Engineering, Department of Plastic Surgery, University of California, Irvine, Orange, CA, United States.
J Plast Reconstr Aesthet Surg. 2019 Jul;72(7):1207-1218. doi: 10.1016/j.bjps.2019.03.008. Epub 2019 Mar 21.
Extracellular vesicles (EVs) are cell-secreted packages that deliver cargo to target cells to effect functional and phenotypic changes. They are secreted by many different cell types, including adipose-derived stem cells (ADSCs), which are a promising field of study in regenerative medicine. Our aim was to perform a systematic review of the literature to summarize the scientific work that has been conducted on ADSC EVs to date.
The Pubmed database was queried with keywords (and variations of) "adipose derived stem cell," "stromal vascular fraction," and "extracellular vesicles." We excluded review papers, then manually screened articles based on title and abstract. Full-text articles were assessed for eligibility to include in final review.
While an extensive body of research exists on EVs, a much smaller proportion of that is original research on ADSC EVs. Of 44 manuscripts that met our database search criteria, 21 articles were selected for our systematic review.
ADSC EVs were found to exert effects on angiogenesis, cell survival and apoptosis, inflammation, tissue regeneration, and reduction of disease pathology. Further studies examine characteristics of ADSC EVs. Future work should aim to further detail the safety profiles of ADSC EVs given their potential for cell-based therapies. The body of research studies characterizing ADSC EVs continues to expand, and much work remains to be done before human pilot studies can be considered. To our knowledge, we offer the first systematic review summarizing the research on ADSC EVs and their determined roles to date.
细胞外囊泡（EVs）是细胞分泌的包裹体，可将物质传递至靶细胞，从而引起功能和表型变化。它们由许多不同类型的细胞分泌，包括脂肪来源干细胞（ADSCs），这是再生医学中一个很有前景的研究领域。我们的目的是对文献进行系统综述，以总结迄今为止关于ADSC衍生细胞外囊泡的科学研究工作。
在PubMed数据库中使用关键词（及其变体）“脂肪来源干细胞”、“基质血管成分”和“细胞外囊泡”进行检索。我们排除了综述论文，然后根据标题和摘要手动筛选文章。对全文进行评估，以确定是否符合纳入最终综述的条件。
虽然关于细胞外囊泡已有大量研究，但其中针对ADSC衍生细胞外囊泡的原创研究比例要小得多。在符合我们数据库搜索标准的44篇手稿中，有21篇文章被选入我们的系统综述。
发现ADSC衍生细胞外囊泡对血管生成、细胞存活与凋亡、炎症、组织再生以及疾病病理的减轻有影响。进一步的研究探讨了ADSC衍生细胞外囊泡的特征。鉴于ADSC衍生细胞外囊泡在基于细胞的治疗中的潜力，未来的工作应旨在进一步详细了解其安全性。对ADSC衍生细胞外囊泡进行特征描述的研究仍在不断扩展，在考虑进行人体初步研究之前，仍有许多工作要做。据我们所知，我们提供了第一篇系统综述，总结了迄今为止关于ADSC衍生细胞外囊泡及其确定作用的研究。
