Department of Agricultural and Food Sciences, Alma Mater Studiorum, University of Bologna, Ozzano dell'Emilia, Bologna 40064, Italy.
Department of Medical Sciences, Microbiology Section, University of Ferrara, Ferrara 44121, Italy.
Poult Sci. 2019 Sep 1;98(9):3602-3610. doi: 10.3382/ps/pez148.
This study investigated for the first time the decontamination efficacy of a probiotic-based cleaning product containing Bacillus subtilis, Bacillus pumilus, and Bacillus megaterium spores on fresh and reused broiler litters during 3 rearing cycles of 6 wk each. Moreover, the impact of reused litters treated with the cleaning product on the chicken caeca microbiota was assessed at the end of the rearing cycles in comparison to untreated litter. The Bacillus spores provided with the cleaning treatment were able to successfully colonize the reused poultry litters, decreasing the mean counts of total aerobic bacteria, Enterobacteriaceae, and coagulase positive Staphylococci. The decrease of Enterobacteriaceae, mainly represented by the genus Escherichia, was also observed in the caeca of broilers reared on reused litters treated with the cleaning product. Moreover, the treatment retained the caeca content of Ruminococcaceae and Faecalibacterium as well as the level of biodiversity among the bacteria genera colonizing the caeca of animals reared on reused litter. Overall, the results of this study highlight a positive effect of the probiotic-based cleaning strategy on the microbial decontamination of reused litters and on broiler caeca stability, thereby enhancing animal health and prevention of poultry diseases.
本研究首次调查了一种基于益生菌的清洁产品对新鲜和重复使用的肉鸡垫料的消毒效果，该清洁产品含有枯草芽孢杆菌、短小芽孢杆菌和巨大芽孢杆菌孢子，在 3 个 6 周的饲养周期中重复使用。此外，还评估了在饲养周期结束时，与未处理的垫料相比，用清洁产品处理过的重复使用垫料对鸡盲肠微生物群的影响。清洁处理中提供的芽孢杆菌孢子能够成功定殖于重复使用的家禽垫料中，降低了总需氧菌、肠杆菌科和凝固酶阳性葡萄球菌的平均计数。在使用清洁产品处理的重复使用垫料饲养的肉鸡盲肠中，也观察到肠杆菌科的减少，主要是大肠杆菌属。此外，该处理保留了盲肠内容物中的 Ruminococcaceae 和 Faecalibacterium 以及定殖于使用重复使用垫料饲养的动物盲肠的细菌属之间的生物多样性水平。总体而言，这项研究的结果突出了基于益生菌的清洁策略对重复使用的垫料的微生物消毒和肉鸡盲肠稳定性的积极影响，从而增强了动物健康和预防家禽疾病的能力。
