沙特阿拉伯盖西姆省皮肤利什曼病患者中利什曼原虫种属的流行率。
Prevalence of Leishmania species among patients with cutaneous leishmaniasis in Qassim province of Saudi Arabia.
机构信息
Department of Medical Biochemistry, College of Medicine, Qassim University, P.O. Box 6655, Buraidah, 51452, Saudi Arabia.
Research Center, College of Medicine, Qassim University, Buraidah, Saudi Arabia.
出版信息
BMC Public Health. 2019 Apr 5;19(1):384. doi: 10.1186/s12889-019-6710-8.
BACKGROUND
Leishmaniasis is a parasitic infection endemic in more than ninety countries of the world. The cutaneous leishmaniasis (CL) is a most common form of leishmaniasis and it remains to be a major public health issue in Saudi Arabia. This study was undertaken to investigate the Leishmania species responsible for CL infection in different provinces of Qassim, Saudi Arabia.
METHODS
Skin biopsies were obtained from CL patients and DNA was extracted using the Magna pure system. Leishmania species were identified by highly specific/sensitive quantitative and qualitative PCR.
RESULTS
Out of total 206 CL biopsies, 49.5% biopsies were found to be positive for Leishmania major (L. major), 28.6% biopsies were positive for Leishmania tropica (L. tropica), 3.9% were found to be positive for Leishmania infantum/donovani (L. infantum/donovani). Not only have these, all tested CL biopsies showed negative test for Leishmania mexicana (L. mexicana) and Leishmania viannia (L. viannia).
CONCLUSIONS
This is the first comprehensive study that shows the majority of CL in Qassim was caused by L. major and L. tropica. To the best of our knowledge, this is the very first report that shows the occurrence of L. infantum/donovani in Saudi Arabia. This requires higher alert to the Ministry of Health of Saudi Arabia to take proactive actions in preventing the onset of L. major, L. tropica, L. infantum and L. donovani infections.
背景
利什曼病是一种寄生虫感染，在世界上 90 多个国家流行。皮肤利什曼病（CL）是最常见的利什曼病形式，在沙特阿拉伯仍然是一个主要的公共卫生问题。本研究旨在调查导致沙特 Qassim 不同省份 CL 感染的利什曼原虫种类。
方法
从 CL 患者中获取皮肤活检，并使用 Magna pure 系统提取 DNA。通过高度特异性/敏感的定量和定性 PCR 鉴定利什曼原虫种类。
结果
在总共 206 例 CL 活检中，49.5%的活检被发现为利什曼原虫（L. major）阳性，28.6%的活检为利什曼原虫（L. tropica）阳性，3.9%的活检为利什曼原虫（L. infantum/donovani）阳性。不仅如此，所有测试的 CL 活检均显示利什曼原虫（L. mexicana）和利什曼原虫（L. viannia）检测均为阴性。
结论
这是第一项全面研究，表明 Qassim 的大多数 CL 是由 L. major 和 L. tropica 引起的。据我们所知，这是首次报道 L. infantum/donovani 在沙特阿拉伯发生。这需要沙特阿拉伯卫生部提高警惕，采取积极行动预防 L. major、L. tropica、L. infantum 和 L. donovani 感染的发生。
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