在 CA1 海马体中过度表达激活的 CaMKII 会损害情境辨别能力，但不会损害情境条件反射。
Overexpression of activated CaMKII in the CA1 hippocampus impairs context discrimination, but not contextual conditioning.
机构信息
School of Biological Sciences, Seoul National University, Gwanak-gu, Seoul, 08826, South Korea.
出版信息
Mol Brain. 2019 Apr 5;12(1):32. doi: 10.1186/s13041-019-0454-3.
Abstract
Calcium/Calmodulin-dependent protein kinase II (CaMKII) plays a key role in the molecular mechanism of memory formation. CaMKII is known to be activated specifically in the activated spines during memory formation. However, it is unclear whether the specific activation of CaMKII is necessary for encoding information. Here, we overexpressed active form of CaMKII (CaMKII*) in the hippocampal CA1 region to activate CaMKII nonspecifically. Moreover, we examined context-discrimination performance of mice. We found that the mice with overexpression of CaMKII* showed impaired context-discrimination ability, while the contextual fear conditioning remained intact. These results indicate that spatial specificity of CaMKII activation is necessary for context discrimination.
摘要
钙/钙调蛋白依赖性蛋白激酶 II（CaMKII）在记忆形成的分子机制中起着关键作用。已知 CaMKII 在记忆形成过程中专门在激活的棘突中被激活。然而，CaMKII 的特异性激活是否对信息编码是必要的尚不清楚。在这里，我们在海马 CA1 区过表达活性形式的 CaMKII（CaMKII*）以非特异性地激活 CaMKII。此外，我们检查了小鼠的情境辨别性能。我们发现，过表达 CaMKII*的小鼠表现出受损的情境辨别能力，而情境恐惧条件反射保持完整。这些结果表明 CaMKII 激活的空间特异性对于情境辨别是必要的。
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